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Factors affecting survival of test bacteria in sea water: marine bacteria, test bacteria and solid surfaces

Faktoren, welche das Überleben von Testbakterien in Meerwasser beeinflussen: Meeresbakterien, Testbakterien und feste Oberflächen

Kurzfassung

Der Einfluß der vorstehend genannten Faktoren wurde auf die Überlebensfähigkeit vonEscherichia coli, Staphylococcus aureus undSerratia marinorubra in Meerwasser untersucht. Aktivitäten mariner Bakterien führten nicht generell zu verstärkter antibakterieller Wirkung rohen Meerwassers. Häufig waren sie für das Überleben vonE. coli undS. aureus förderlich. Inaktivierte Zellen vonE. coli undS. aureus erhöhten die bakterizide Wirkung rohen und filtersterilisierten Meerwassers gegenüber sekundär inokulierten, gleichartigen Testbakterien, während sie die inaktivierende Potenz autoklavierten Meerwassers verminderten. Durch erhöhtes Angebot an Glasoberfläche/Volumeneinheit, welches die adsorptive Anreicherung organischer Substanzen verstärkt, wurde die Inaktivation vonE. coli undS. aureus meistens beschleunigt, während sich diejenige vonS. marinorubra um so stärker verminderte, je größer das Verhältnis Oberfläche/Volumen war. Raschere Abtötung vonE. coli undS. aureus in Sterilfiltraten als in rohem Meerwasser trat bei erhöhtem Oberfläche/Volumen-Verhältnis häufiger auf als unter Standardbedingungen. Aus den Ergebnissen wird geschlossen, daß die während der Versuche eintretenden Veränderungen des Nährstoffangebotes, hervorgerufen durch Nährstoffverbrauch sowie durch Lysis inaktivierter Testbakterien, bezüglich der bakteriziden Wirkung von Meerwasser generell von größerer Bedeutung sind als bakterizide Stoffwechselprodukte mariner Bakterien.

Summary

1. The antibacterial activity of raw sea water varied considerably during incubation of successive inocula ofEscherichia coli, Staphylococcus aureus, andSerratia marinorubra. In most cases inactivation of second inocula was stronger than that of first ones. However, withS. aureus, contradictory results were obtained also.

2. The bactericidal effect of filter-sterilized sea water was strengthened by inactivated cells ofEscherichia coli andStaphylococcus aureus. Contradictory findings were obtained from autoclaved sea water.

3. Inactivation of test bacteria was greatly influenced by solid surfaces. In most cases, the kill ofEscherichia coli andStaphylococcus aureus in raw and sterile-filtered sea water was stronger at increased surface/volume ratios than under standard conditions. More rapid inactivation of these test strains in sterile-filtered, than in raw, sea water occurred more often at enlarged ratios of solid surface per unit volume. The survival ofSerratia marinorubra was positively affected by solid surfaces.

4. It is concluded that changes in nutritive conditions occurring during the experiments are more important in regard to antibacterial activity of sea water than production of harmful matter by marine bacteria.

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Moebus, K. Factors affecting survival of test bacteria in sea water: marine bacteria, test bacteria and solid surfaces. Helgolander Wiss. Meeresunters 23, 271–285 (1972). https://doi.org/10.1007/BF01609679

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