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The quantitative separation of meiofauna

Die quantitative Isolierung der Meiofauna. Ein Methodenvergleich

A comparison of methods

Kurzfassung

Im Mai 1972 fand an der Meeresstation der Biologischen Anstalt Helgoland ein Arbeitstreffen mit dem Ziel statt, die verschiedenen Methoden zur quantitativen Isolierung der Meiofauna aus dem Sediment in ihrer Effektivität miteinander zu vergleichen. Die Verfahren zur Anreicherung der Meiofauna in der Probe, des Sortierens und Auszählens werden beschrieben und die durch die Teilnehmer am Arbeitstreffen gemeinsam erzielten Ergebnisse diskutiert. Die Wertung der einzelnen Methoden zur Erfassung der Gesamtfauna, der „harten“ und der „weichen“ Meiofauna, von konservierten und nichtkonservierten Organismen sowie die Anwendung der Methoden auf verschiedenen Sedimenttypen werden durch statistische Analysen abgesichert. Eine Zusammenfassung der Arbeitsverfahren sowie deren Charakterisierung und Leistungsfähigkeit beschließt die Darstellung.

Summary

1. This paper presents the results of a meeting on the evaluation of quantitative procedures for the separation of meiofauna, held at the Marine Station of the Biologische Anstalt Helgoland, in May 1972. Close co-operation on the part of the participants (p. 194) has allowed assessment of advantages and disadvantages of the separation methods applied. The time needed for preserved methods can be reduced by changing to scanning rather than picking out for sorting and counting.

2. Sorting without concentrations: This method is suitable for very fine-grained sediments, oozes; it is generally applied for preserved samples; adequate for hard fauna; insufficient for soft fauna; very time consuming.

3. Decantation: Suitable for sandy sediments; generally applied for preserved samples; adequate for hard fauna; insufficient for soft fauna; time consuming.

4. Elutriation: Suitable for sandy sediments; for unpreserved samples only with anaesthetization, more effective with preserved samples; limited for total live fauna. With preserved samples, adequate for hard fauna; insufficient for soft fauna; quick method.

5. Warm-water elutriation: Suitable for sandy sediments; designed for live hard fauna (especially nematodes, ostracods); quick method.

6. Sea-water ice treatment: Suitable for sandy sediments with microporal structure; only for live extraction; limited for hard fauna; well suited for soft fauna (including ciliates and flagellates); time consuming.

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Uhlig, G., Thiel, H. & Gray, J.S. The quantitative separation of meiofauna. Helgolander Wiss. Meeresunters 25, 173–195 (1973). https://doi.org/10.1007/BF01609968

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