- Published:
Amino-acid absorption by developing herring eggs
Helgoländer wissenschaftliche Meeresuntersuchungen volume 29, pages 464–472 (1977)
Abstract
14C-glycine absorption by eggs of the herringClupea harengus from a 2 µM solution at 15°C depends on the stage of embryonic development. Unidirectional14C-glycine influx rates are small at early stages: 0.6 ± 0.1 and 0.5 ± 0.1 pmoles egg−1 h−1 in embryos 5 h and 28 h after fertilization, respectively. They increase drastically about 51 h after fertilization (prior to blastopore closure) to 3.7 ± 0.9 pmoles egg−1 h−1. Glycine uptake steadily continues to increase almost until hatching (maximum values = 18.8 ± 2.7 pmoles egg−1 h−1), decreasing slightly prior to hatching. Distribution ratios (radioactivity µl−1 of egg volume: radioactivity µl−1 ambient medium) exceed the equilibrium ratio of 1 between 51 h and 78 h after fertilization, reaching values of 4.7 two days prior to hatching, thus suggesting the presence of a transport mechanism capable of transferring the amino acid against the concentration gradient. Curves for concentration-dependent14C-glycine and14C-α-aminoisobutyric acid absorption are very similar; they consist of a linear portion at higher concentrations and a saturable component, indicating a mediated uptake process. Calculations performed by means of aminoacid absorption rates and O2 uptake data suggest that herring eggs scarcely obtain nutritional benefits from absorption of dissolved amino acids in natural spawning areas.
Literature cited
Ahearn, G. A. & Townsley, S. J., 1975. Integumentary amino acid transport and metabolism in the apodous sea cucumber,Chiridota rigida. J. exp. Biol.62 733–752.
Christensen, H. N., 1975. Biological transport. Benjamin, Reading, Mass. 514 pp.
Fry, B. J. & Gross, P. R., 1970. Patterns and rates of protein synthesis in sea urchin embryos. I. Uptake and incorporation of amino acids during the first cleavage cycle. Devl. Biol.21 105–124.
Jørgensen, C. B., 1976. August Pütter, August Krogh, and modern ideas on the use of dissolved organic matter in aquatic environments. Biol. Rev.51 291–328.
North, B. B., 1975. Primary amines in California coastal waters: utilization by phytoplankton. Limnol. Oceanogr.20 20–27.
Rosenthal, H., 1968. Schwimmverhalten und Schwimmgeschwindigkeit bei den Larven des HeringsClupea harengus. Helgoländer wiss. Meeresunters.18 453–486.
—— & Stelzer, R., 1970. Wirkungen von 2,4- und 2,5-Dinitrophenol auf die Embryonalentwicklung des HeringsClupea harengus. Mar. Biol.5 325–336.
—— & Fonds, M., 1973. Biological observations during rearing experiments with the garfishBelone belone. Mar. Biol.21 203–218.
Sepers, A. B. J., 1977. The utilization of dissolved organic compounds in aquatic environments. Hydrobiologia52 39–54.
Shick, J. M., 1975. Uptake and utilization of dissolved glycine byAurelia aurita Scyphistomae: temperature effects on the uptake process; nutritional role of dissolved amino acids. Biol. Bull. mar. biol. Lab., Woods Hole148 117–140.
Siebers, D., 1976. Absorption of neutral and basic amino acids across the body surface of two annelid species. Helgoländer wiss. Meeresunters.28 456–466.
—— & Bulnheim, H.-P., 1976. Salzgehaltsabhängigkeit der Aufnahme gelöster Aminosäuren bei dem OligochaetenEnchytraeus albidus. Verh. dt. zool. Ges.,69 212.
Stelzer, R., Rosenthal, H. & Siebers, D. 1971. Einfluß von 2,4-Dinitrophenol auf die Atmung und die Konzentration einiger Metabolite bei Embryonen des HeringsClupea harengus. Mar. Biol.11 369–378.
Stephens, G. C., 1975. Uptake of naturally occurring primary amines by marine annelids. Biol. Bull. mar. biol. Lab., Woods Hole149 397–407.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Siebers, D., Rosenthal, H. Amino-acid absorption by developing herring eggs. Helgolander Wiss. Meeresunters 29, 464–472 (1977). https://doi.org/10.1007/BF01609984
Issue Date:
DOI: https://doi.org/10.1007/BF01609984