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Malate dehydrogenase and tetrazolium oxidase of scyphistomae ofAurelia aurita, Chrysaora quinquecirrha, andCyanea capillata (Scyphozoa: Semaeostomeae)
Malatdehydrogenase und Tetrazoliumoxydase der Scyphistomae vonAurelia aurita, Chrysaora quinquecirrha andCyanea capillata (Scyphozoa: Semaeostomeae)
Helgoländer wissenschaftliche Meeresuntersuchungen volume 25, pages 206–213 (1973)
Kurzfassung
Im Gewebe der Scyphistomae vonAurelia aurita, Chrysaora quinquecirrha undCyanea capillata wurde das Isoenzymmuster der Malatdehydrogenase (MDH) und der Tetrazoliumoxydase (TO) durch Anwendung der Polyacrylamidgel-Elektrophorese bestimmt. Entsprechend der Reihenfolge der genannten Arten betrug die Anzahl der gefundenen MDH-Isoenzymbanden 4,5 bzw. 1, während sich die der TO-Isoenzymbanden auf 2,1 bzw. 1 belief. Es wird darauf hingewiesen, daß das Isoenzymmuster für die taxonomische Zuordnung der schwer zu unterscheidenden Scyphopolypen neben anderen Merkmalen mit herangezogen werden kann.
Summary
1. The malate dehydrogenase (MDH) isozyme patterns of the three most common polyps of Chesapeake Bay (USA) differ in number and in electrophoretic mobilities:Aurelia aurita: 4 bands (Rm=0.29, 0.33, 0.39, 0.43);Chrysaora quinquecirrha: 5 bands (Rm=0.21, 0.22, 0.40, 0.53, 0.54);Cyanea capillata: 1 band (Rm=0.50) or sometimes a second (Rm=0.25).
2. The tetrazolium oxidase (TO) isozyme patterns differ as follows:Aurelia aurita: 2 bands (Rm=0.39, 0.51);Chrysaora quinquecirrha: 1 band (Rm=0.58);Cyanea capillata: 1 band (Rm=0.39).
3. Isozyme components of MDH and TO, either singly or in combination, may be used to distinguish the common Chesapeake Bay polyps of unknown origin.
Literature cited
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Contribution No. 547 from the Virginia Institute of Marine Science.
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Lin, A.L., Zubkoff, P.L. Malate dehydrogenase and tetrazolium oxidase of scyphistomae ofAurelia aurita, Chrysaora quinquecirrha, andCyanea capillata (Scyphozoa: Semaeostomeae). Helgolander Wiss. Meeresunters 25, 206–213 (1973). https://doi.org/10.1007/BF01611197
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DOI: https://doi.org/10.1007/BF01611197