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The extraction of glycogen from marine invertebrate tissues

Die Glykogenextraktion aus dem Gewebe mariner Invertebraten

Kurzfassung

Es wird der Nachweis geführt, daß die Glykogenextraktion aus den Geweben einiger mariner Invertebraten(Balanus balanoides, Nephtys hombergi, Carcinus maenas, Mytilus edulis) durch 5% Trichloressigsäure von dem Homogenisierungsverfahren abhängt. In Übereinstimmung mit Untersuchungen an Rattengewebe können die Glykogenfraktionen nicht durch ihre Löslichkeit in kalter 5% Trichloressigsäure unterschieden werden. Daher ist nur die Bestimmung des Gesamt-Glykogengehaltes angebracht.

Summary

1. It is shown that the extraction by 5% trichloracetic acid of glycogen from the tissues of some typical marine invertebrates(Balanus balanoides, Nephtys hombergi, Carcinus maenas, Mytilus edulis) is a function of the homogenization procedure.

2. In agreement with work on rat tissues, glycogen fractions cannot be differentiated by their solubility in cold 5% trichloracetic acid. Total glycogen only need be determined.

Literature cited

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Barnes, H., Heath, J.R. The extraction of glycogen from marine invertebrate tissues. Helgolander Wiss. Meeresunters 13, 115–117 (1966). https://doi.org/10.1007/BF01612660

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  • DOI: https://doi.org/10.1007/BF01612660

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