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Isolierung von gelösten Kohlenhydraten aus dem Meerwasser

Isolation of dissolved carbohydrates from sea water

Helgoländer wissenschaftliche Meeresuntersuchungen volume 12, pages 253–259 (1965)Cite this article

Abstract

The carbohydrates dissolved in sea water have been mostly determined in terms of total concentration. Only in a few cases have they been identified and single components measured quantitatively. In the present work we have studied the optimal conditions for the isolation of soluble sugars from sea water by means of carbon adsorption. One to 2 liters of sea water are passed through 13,8 × 4 cm carbon-celite-columns, salts are washed out with 400 ml of distilled water and sugars eluted with 400 ml of 20 % aethanol. The eluted sugar fraction is concentrated in vacuo, and the isolated sugars may be determined and identified by means of analytical micromethods. Water samples from several stations of the Deutsche Bucht (Southern North Sea) were collected, filtrated and analysed by the above mentioned method. Rhamnose, ribose, sucrose and one not yet identified sugar were isolated. The carbon adsorption method might be of interest also for limnological studies.

Zusammenfassung

1. Der Wert der summarischen Bestimmung der Gesamtmenge von im Meerwasser gelösten Kohlenhydraten wird diskutiert.

2. Die optimalen Versuchsbedingungen für die Isolierung von gelösten Kohlenhydraten aus 1 bis 2 l Meerwasser mit Hilfe des Kohleadsorptionsverfahrens werden mitgeteilt. In dem salzfreien Eluat der Kohle-Celite-Säulen können die einzelnen Zucker mit Mikronachweisverfahren quantitativ analysiert werden.

3. Aus Seewasserproben (Deutsche Bucht) wurden mit der geschilderten Methode Rhamnose, Ribose, Saccharose und ein noch nicht identifizierter Zucker isoliert.

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  1. Heinz Schaefer

    Present address: , Maximilianstr. 43-45, 673 Neustadt/Weinstr.

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  1. Biologische Anstalt Helgoland, Meeresstation, Helgoland

    Heinz Schaefer

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  1. Heinz Schaefer
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Der Deutschen Forschungsgemeinschaft danke ich für die Unterstützung der Arbeit.

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Schaefer, H. Isolierung von gelösten Kohlenhydraten aus dem Meerwasser. Helgolander Wiss. Meeresunters 12, 253–259 (1965). https://doi.org/10.1007/BF01880934

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Helgoland Marine Research

ISSN: 1438-3888

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