Skip to main content
Download PDF
  • Fishes
  • Published:

Isolation of infectious pancreatic necrosis virus (serotype Ab) from diverse species of estuarine fish

Helgoländer Meeresuntersuchungen volume 37, pages 317–328 (1984)Cite this article

Abstract

Two significant fish kills occurred in the Pamlico River estuary (North Carolina, USA), one in December 1981 and January 1982, and the other in June 1982. The first involved only the southern flounder(Paralichthys lethostigma). Histopathologic examination of morbid and moribund flounder revealed extensive sloughing and necrosis of the mucosa of the pyloric caeca and intestine, and inflammation of the submucosa of the pyloric caeca. Brain and internal organ homogenates from morbid and moribund flounder were assayed on CHSE-214 cells, and a virus was isolated. Virus titers ranged from≤8.4 · 102 to 6.3 · 107 TCID50 per gram of tissue. Cross-plaque neutralization assays indicated that the southern flounder virus was infectious pancreatic necrosis virus serotype Ab. Immersion challenge showed the isolate is only slightly virulent for fry of brook trout(Salvelinus fontinalis). The second fish kill involved the southern flounder and six other species: hogchoker(Trinectes maculatus), Atlantic silverside(Menidia menidia), spot(Leiostomus xanthurus), Atlantic croaker(Micropogon undulatus), silver perch(Bairdiella chrysura), and striped mullet(Mugil cephalus). Virus was isolated from southern founder, hogchoker, Atlantic silverside, and spot. Neutralization assays indicated that the four isolates were nearly identical; however, the diversity of species affected suggests that the virus might not have been the specific cause of mortality.

Literature cited

  • Archetti, I. & Horsfall, F. L., 1950. Persistent antigenic variation of influenza A viruses after incomplete neutralization in vivo with heterologous immune serum. — J. exp. Med.92, 411–462.

    Article  Google Scholar 

  • Dorson, M., 1983. Infectious pancreatic necrosis of salmonids. In: Antigens of fish pathogens. Ed. by D. P. Anderson, M. Dorson & Ph. Dubourget. Fondation Marcel Merieux, Lyon, 7–32.

    Google Scholar 

  • Helms, R. F., 1981. Pamlico river flounder fish kill (an investigation). North Carolina Dept. Nat. Resourc. & Community Devt, Raleigh, N. C., 28 pp.

    Google Scholar 

  • Hill, B. J., 1976. Properties of a virus isolated from the bivalve molluscTellina tenuis (Da Costa). In: Wildlife diseases. Ed. by L. A. Page. Plenum Press, New York, 701 pp.

    Google Scholar 

  • Hill, B. J., 1982. Infectious pancreatic necrosis virus and its virulence. In: Microbial diseases of fish. Ed. by R. J. Roberts. Acad. Press, London, 91–114.

    Google Scholar 

  • Kärber, G., 1931. Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. — Arch. Pathol. Pharmakol.162, 480–483.

    Article  Google Scholar 

  • Macdonald, R. D. & Yamamoto, T., 1977. The structure of infectious pancreatic necrosis virus RNA. — J. gen. Virol.34, 235–247.

    PubMed  Google Scholar 

  • McAllister, P. E., 1983. Infectious pancreatic necrosis (IPN) of salmonid fishes. — Fish Dis. Leafl. U.S. Fish Wildl. Serv.65, 1–12.

    Google Scholar 

  • McAllister, P. E., Owens, W. J. & Bowling, D. E., 1983. Infectious pancreatic necrosis virus: efficacy of vaccine preparations. — 8th Annual Eastern Fish Health Workshop, Kearneysville, West Virginia. 21–23 June 1983, 22. (Abstract).

  • McDaniel, D. (Ed.), 1979. Procedures for the detection and identification of certain fish pathogens. Am. Fish. Soc., Fish Health Sect., Washington, D. C., 118 pp.

    Google Scholar 

  • Okamoto, N., Sano, T., Hedric, R. P. & Fryer, J. L., 1983. Antigenic relationships of selected strains of infectious pancreatic necrosis virus and European eel virus. — J. Fish Dis.6, 19–25.

    Google Scholar 

  • Stephens, E. B., Newman, M. W., Zachary, A. L. & Hetrick, F. M., 1980. A viral aetiology for the annual spring epizootics of Atlantic menhadenBrevoortia tyrannus (Latrobe) in Chesapeake Bay. — J. Fish Dis.3, 387–398.

    Google Scholar 

  • Underwood, B. O., Smale, C. J. & Brown, F., 1977. Relationships of a virus fromTellina tenuis to infectious pancreatic necrosis virus. — J. gen. Virol.36, 93–109.

    PubMed  Google Scholar 

  • Wolf, K. & Quimby, M. C., 1973. Fish viruses: buffers and methods for plaquing eight agents under normal atmosphere. — Appl. Microbiol.25, 659–664.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Box 700, 25430, Kearneysville, West Virginia, USA

    P. E. McAllister & W. J. Owens

  2. National Marine Fisheries Service, Northeast Fisheries Center, 21654, Oxford, Maryland, USA

    M. W. Newman

  3. North Carolina Department of Natural Resources, and Community Development, Division of Natural Resources, 27611, Raleigh, North Carolina, USA

    J. H. Sauber

Authors
  1. P. E. McAllister
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. W. Newman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. H. Sauber
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. J. Owens
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

McAllister, P.E., Newman, M.W., Sauber, J.H. et al. Isolation of infectious pancreatic necrosis virus (serotype Ab) from diverse species of estuarine fish. Helgolander Meeresunters 37, 317–328 (1984). https://doi.org/10.1007/BF01989314

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01989314

Keywords

Helgoland Marine Research

ISSN: 1438-3888

Contact us