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Isolation and characterization of a new subspecies ofMycobacterium chelonei infectious for salmonid fish
Helgoländer Meeresuntersuchungen volume 37, pages 329–342 (1984)
Abstract
Rapidly growing, nonchromogenic mycobacteria were isolated from salmonid fish at five locations in the states of Oregon and Montana, USA. The isolates were characterized by biochemical, physiological, genetic and mycolic acid properties, then subjected to taxonomic analysis. Detection of mycobacterial mycolic acids and a percent guanine plus cytosine value of 63 ± 1.7 mol% confirmed that the isolates belong to the genusMycobacterium. The internal similarity of the isolates was 94.2 ± 3.4 %. None of the isolates grew at 37 °C. A comparison of their properties with those of other rapidly growing, nonchromogenic and photochromogenic mycobacteria was made. The salmonid isolates showed a relationship toM. chelonei subspecieschelonei andM. chelonei subspeciesabscessus, but had biochemical properties which were intermediate to these two subspecies. Acid methanolysates of the salmonid isolates, analyzed by two dimensional thin-layer chromatography, produced lipid patterns identical to those of both subspecies ofM. chelonei. Sufficient differences in biochemical properties and the inability to grow at 37 °C suggest these isolates be regarded as a new subspecies ofM. chelonei. We propose the nameM. chelonei subspeciespiscarium subsp. nov. (L. adj.piscarius of fish). The isolates were not infectious for mice. Experimental infections were produced in juvenile salmonid fish. The occurrence of mycobacterial infections in selected salmonid populations from Oregon hatcheries and the Pacific Ocean ranged from 0 to 26 %.
Literature cited
Ashburner, L. D., 1977. Mycobacteriosis in hatchery-confined chinook salmon (Oncorhynchus tshawytscha Walbaum) in Australia. — J. Fish Biol.10, 523–528.
Banner, C. R., Rohovec, J. S. & Fryer, J. L., 1982. A rapid method for labeling rabbit immunoglobulin with fluorescein for use in detection of fish pathogens. — Bull. Europ. Fish Path.2, 35–37.
Bönicke, R., 1962. Identification of mycobacteria by biochemical methods. — Bull. int. Un. Tuberc.32, 13–68.
Frost, G. D., 1968. Oral immunization for possible control of furunculosis in fish. Masters Thesis, Oregon State Univ., Corvallis, Oregon, 59 pp.
Fryer, J. L. & Sanders, J. E., 1981. Bacterial kidney disease of salmonid fish. — A. Rev. Microbiol.35, 273–298.
Goodfellow, M. & Wayne, L. G., 1982. Taxonomy and nomenclature. In: The biology of the mycobacteria. Ed. by C. Ratledge & J. L. Stanford. Acad. Press, New York,1, 471–521.
Gordon, R. E. & Mihm, J. M., 1959. A comparison of four species of mycobacteria. — J. gen. Microbiol.21, 736–748.
Hanson, R. S. & Phillips, J. A., 1981. Chemical composition. In: Manual of methods for general bacteriology. Ed. by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Am. Soc. Microbiol., Washington, D. C., 328–392.
Johnson, J. L., 1981. Genetic characterization. In: Manual of methods for general bacteriology. Ed. by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Am. Soc. Microbiol., Washington, D. C., 450–472.
Kanetsuna, F. & Bartoli, A., 1972. A simple chemical method to differentiateMycobacterium fromNocardia. — J. gen. Microbiol.70, 209–212.
Kubica, G. P., Baess, I., Gordon, R. E., Jenkins, P. A., Kwapinski, J. B. G., McDurmont, C., Pattyn, S. R., Saito, H., Silcox, V., Stanford, J. L., Takeya, K. & Tsukamura, M., 1972. A cooperative numerical analysis of rapidly growing mycobacteria. — J. gen. Microbiol.73, 55–70.
Kubica, G. P. & Vestal, A. L., 1961. The arylsulfatase activity of acid-fast bacilli. I. Investigation of activity of stock cultures of acid-fast bacilli. — Am. Rev. Resp. Dis.83, 728–732.
Mandel, M. L., Igambi, L., Bergendahl, J., Dodson, M. L. & Scheltgen, E., 1970. Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. — J. Bact.101, 333–338.
Marks, J., 1976. A system for the examination of tubercle bacilli and other mycobacteria. — Tubercle, Lond.57, 207–225.
Matthaei, E., 1950. Simplified fluorescence microscopy of tubercle bacilli. — J. gen. Microbiol.9, 393–398.
Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M., 1980. Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. — J. Chromat.188, 221–233.
Minnikin, D. E., Minnikin, S. M., Goodfellow, M. & Stanford, J. L., 1982. The mycolic acids ofMycobacterium chelonei. — J. gen. Microbiol.128, 817–822.
Mizuguchi, Y. & Tokunaga, T., 1970. Method for isolation of deoxyribonucleic acid from mycobacteria. — J. Bact.104, 1020–1021.
Paik, G. & Suggs, M. T., 1974. Reagents, stains, and miscellaneous test procedures. In: Manual of clinical microbiology. Ed. by E. H. Lennette, E. H. Spaulding, J. P. Truant. Am. Soc. Microbiol., Washington, D. C., 930–950.
Parisot, T. J., 1958. Tuberculosis of fish. A review of the literature with a description of the disease in salmonid fish. — Bact. Rev.22, 240–245.
Ross, A. J., 1960.Mycobacterium salmoniphilium sp. nov. from salmonid fishes. — Am. Rev. Resp. Dis.81, 241–250.
Ross, A. J., 1970, Mycobacteriosis among salmonid fishes. In: A Symposium on diseases of fishes and shellfishes. Ed. by S. F. Snieszko. Am. Fish. Soc., Washington, D. C., 279–283 (Spec. Publ. Am. Fish. Soc. 5).
Ross, A. J., Earp, B. & Wood, J. W., 1959. Mycobacterial infections in adult salmon and steelhead trout returning to the Columbia River Basin and other areas in 1957. — Spec. scient. Rep. U. S. Fish. Wildl. Serv.332, 1–34.
Runyon, E. H., Selin, M. J. & Harris, H. W., 1959. Distinguishing mycobacteria by the niacin test. A modified procedure. — Am. Rev. Resp. Dis.79, 663–665.
Runyon, E. H., Wayne, L. G. & Kubica, G. P., 1974. Family II.Mycobacteriaceae Chester 1897, 63. In: Bergey's manual of determinative biology. Ed. by R. E. Buchanan & N. E. Gibbons. Williams & Wilkins, Baltimore, 681–701.
Saito, H., Gordon, R. E., Juhlin, I., Kappler, W., Kwapinski, J. B. G., McDurmont, C., Pattyn, S. R., Runyon, E. H., Stanford, J. L., Tarnok, I., Tasaka, H., Tsukamura, M. & Weiszfeiler, J., 1977. Cooperative numerical analysis of rapidly growing mycobacteria. The second report. — Int. J. syst. Bact.27, 75–85.
Stanford, J. L. & Beck, A., 1969. Bacteriological serological studies of fast growing mycobacteria identified asMycobacterium friedmanii. — J. gen. Microbiol.58, 99–106.
Tsukamura, M., 1961. Certain saprophytic mycrobacteria from soil sources forming a red color product from PAS. — Am. Rev. Resp. Dis.84, 916.
Tsukamura, M., 1965. Salicylate degradation test for differentiation ofMycobacterium fortuitum from other mycobacteria. — J. gen. Microbiol.41, 309–315.
Tsukamura, M., 1966. Adansonian classification of mycobacteria. — J. gen. Microbiol.15, 253–273.
Tsukamura, M., 1967a. Differentiation of mycobacteria by utilization of nitrogen compounds as simultaneous nitrogen and carbon sources. — Am. Rev. Resp. Dis.95, 307–310.
Tsukamura, M., 1967b. Identification of mycobacteria. — Tubercle, Lond.48, 311–338.
Tsukamura, M., 1981. Numerical analysis of rapidly growing, nonphotochromogenic mycobacteria, includingMycobacterium agri (Tsukamura 1972) Tsukamura sp. nov., nom. rev. — Int. J. syst. Bact.31, 247–258.
Wayne, L. G., Engel, H. W. B., Grassi, C., Gross, W., Hawkins, J., Jenkins, P. A., Kappler, W., Kleeberg, H. H., Krasnow, I., Nel, E. E., Pattyn, S. R., Richards, P. A., Showalter, S., Slosarek, M., Szabo, I., Tarnok, I., Tsukamura, M., Vergmann, B. & Wolinsky, E., 1976. Highly reproducible techniques for use in systematic bacteriology in the genusMycobacterium: tests for niacin and catalase and for resistance to isoniazid, thiophene 2-carboxylic acid hydrazide, hydroxylamine, and p-nitrobenzoate. — Int. J. syst. Bact.26, 311–318.
Wood, J. W. & Ordal, E. J., 1958. Tuberculosis in Pacific salmon and steelhead trout. — Contr. Fish Commn Ore25, 1–38.
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Arakawa, C.K., Fryer, J.L. Isolation and characterization of a new subspecies ofMycobacterium chelonei infectious for salmonid fish. Helgolander Meeresunters 37, 329–342 (1984). https://doi.org/10.1007/BF01989315
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DOI: https://doi.org/10.1007/BF01989315