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Invertebrate bioassays with North Sea water samples. I. Structural effects on embryos and larvae of serpulids, oysters and sea urchins
Helgoländer Meeresuntersuchungen volume 39, pages 1–19 (1985)
Abstract
Structural effects of bottom and surface water samples from two dumping grounds in the inner German Bight on the development of three meroplanktonic organisms(Pomatoceros triqueter: Polychaeta,Psammechinus miliaris: Echinodermata andCrassostrea gigas, Mollusca) were investigated. The titaniumdioxide dumping site was sampled immediately after dumping (within the visible waste trail 1 km behind the vessel), and 10 h after dumping. Samples were taken in the sewage sludge deposition area in the intervals between the usual dumping activities, regardless of the exact dumping schedule. The preserved bioassay test organisms were inspected microscopically to count percentages of “normal” larval hatch in test water samples, reference water samples and laboratory aged control water samples (5 to 10 replicates). The relative water quality at various dumping sites was expressed in terms of “net risk”-values (Woelke, 1972) compared to hatching rates observed in the controls. Larval development ofP. triqueter was significantly suppressed (up to −22 % “net risk”) in trail water of the titanium dioxide dump site while the development of sea urchin larvae was still affected in the 10 h surface samples. Hatching of all test organisms in bottom-water samples from the centre of the sewage sludge dump site was affected to different degrees when compared to reference areas about 4 km north or 6 km northwest of the dumping area. The general usefulness of standardized bioassay procedures in pollution monitoring programmes is discussed. The results presented here call for further verification to minimize experimental background variability and to enlarge the catalogue of suitable effects criteria.
Literature cited
Bay, S. M., Oshida, P. S. & Jenkins, K. D., 1983. A simple new bioassay based on echinochrome synthesis by larval sea urchins. — Mar. environ. Res.8 29–39.
Bougis, P., 1967. Utilisation des plutéus en écologie expérimentale. — Helgoländer wiss. Meeresunters.15 59–68.
Bougis, P., Corre, M. C. & Etienne, M., 1979. Sea urchin larvae as a tool for assessment of the quality of sea water. — Annls Inst. oceanogr., Paris55 21–26.
Bourne, N., Rogers, H., Mahood, H. & Neil, D., 1981. Water quality study in Lady Smith Harbour, British Columbia. — Can. tech. Rep. Fish. aquat. Sci.1026 1–57.
Calabrese, A., Collier, R. S., Nelson, D. A. & MacInnes, J. R., 1973. The toxicity of heavy metals to embryos of the American oysterCrassostrea virginica. — Mar. Biol.18 162–166.
Cardwell, R. D., Woelke, C. E., Carr, M. J. & Sanborn, E. W., 1977. Evaluation of the efficacy of sulfite pulp mill pollution abatement using oyster larvae. In: Aquatic toxicology and hazard evaluation. Ed. by F. L. Mayer & J. L. Hamelink. American Society for Testing and Materials, Philadelphia, 281–295.
Eagle, R. A., Hardiman, P. A., Norton, M. G. & Nunny, R. S., 1979. The field assessment of effects of dumping wastes at sea: 4. A survey of the sewage sludge disposal area off Plymouth. — Fish. Res. tech. Rep.50 1–28.
Falk-Petersen, I.-B., 1979. Toxic effects of aqueous extracts of Ekofisk crude oil, crude oil fractions, and commercial oil products on the development of sea urchin eggs. — Sarsia64 161–169.
Gray, J. S. & Pearson, T. H., 1982. Objective selection of sensitive species indicative of pollution-induced change in benthic communities. I. Comparative methodology. — Mar. Ecol.9 111–119.
Greenwood, P. J., 1980. Possible uses of the developmental embryology of the sea urchinParechinus angulosus for pollution monitoring in the nearshore marine environment. Ph. D. thesis, Univ. of Cape Town, 334 pp.
Greenwood, P. J., & Brown, A. C., 1974. Effect of ammonium nitrate solutions on fertilization and development of the sea urchinParechinus angulosus. — Zool. Afr.9 205–209.
ICES, 1976. ICES working group on pollution baseline and monitoring studies in the Oslo Commission and ICNAF areas. Report of the sub-group on the feasibility of effects monitoring. — C. M./ICES,E 44 1–36.
ICES, 1978. On the feasibility of effects monitoring. — Coop. Res. Rep.75 1–42.
Karbe, L., Borchardt, T., Dannenberg, R. & Meyer, E., 1984. Ten years of experience using marine and freshwater hydroid bioassays. In: Ecotoxicological testing for the marine environment. Ed. by G. Persoone, E. Jaspers, & C. Claus. Lab. Biol. Res. Aquat. Pollut., State Univ. Ghent, Ghent,2, 99–129.
Klöckner, K., 1978. Zur Ökologie vonPomatoceros triqueter (Serpulidae, Polychaeta). II. Einflüsse der Temperatur auf Toleranz, Tubusregeneration, Sauerstoffverbrauch und Filtrierleistung. — Helgoländer wiss. Meeresunters.31 257–284.
Kobayashi, N., 1971. Fertilized sea urchin eggs as an indicatory material for marine pollution bioassay, preliminary experiments. — Publs Seto mar. biol. Lab.18 379–406.
Kobayashi, N., 1981. Comparative toxicity of various chemicals, oil extracts and oil dispersant extracts to Canadian and Japanese sea urchin eggs. — Publs Seto mar. biol. Lab.26 123–133.
Kobayashi, N., 1984. Marine ecotoxicological testing with echinoderms. In: Ecotoxicological testing for the marine environment. Ed. by G. Persoone, E. Jaspers, & C. Claus. Lab. Biol. Res. Aquat. Pollut., State Univ. Ghent, Ghent,1, 341–405.
Lloyd, R. & Thain, J. E., 1981. The use of the oyster bioassay to monitor coastal water quality. — ICESWG MPNA 5(1/2, 1–4.
Lönning, S., 1977. The sea urchin egg as a test object in oil pollution studies. — Rapp. P.-v. Réun. Cons. int. Explor. Mer171 186–188.
Martin, M., Osborne, K. E., Billig, P. & Glickstein, N., 1981. Toxicities of ten metals toCrassostrea gigas andMytilus edulis embryos andCancer magister larvae. — Mar. Pollut. Bull.12 305–308.
Nelson, D., Miller, J., Pereira, J. & Calabrese, A., 1983. Monitoring water quality at a dredge spoil dump site using oyster larvae. — C. M./ICES,E 59 1–9.
Okubo, K. & Okubo, T., 1962. Study on the bioassay method for the evaluation of water pollution. II. Use of the fertilized eggs of sea urchins and bivalves. — Bull. Tokai reg. Fish. Res. Lab.32 131–140.
Oshida, P. S., Bay, S. M., Haeckl, A., Goochey, T. K. & Greenstein, D., 1982. Sea water and waste water toxicity studies. — Coast. Wat. Res. Proj. biann. Rep.1981–1982 217–223.
Pearson, T. H., 1975. The benthic ecology of Loch Linnhe and Loch Eil, a sea-loch system on the west coast of Scotland. 4. Changes in the benthic fauna attributable to organic enrichment. — J. exp. mar. Biol. Ecol.20 1–41.
Rachor, E., 1977. Faunenverarmung in einem Schlickgebiet in der Nähe Helgolands. — Helgoländer wiss. Meeresunters.30 63–651.
Sokal, R. R. & Rohlf, F. J., 1969. Biometry. Freeman, San Francisco, 776 pp.
Stebbing, A. R. D., Akesson, B., Calabrese, A., Gentile, J. M., Jensen, A. & Lloyd, R., 1980. The role of bioassays in marine pollution monitoring. — Rapp. P.-v. Réun. Cons. int. Explor. Mer179 322–332.
Stebbing, A. R. D. & Brown, B. E., 1984. Marine ecotoxicological tests with coelenterates. In: Ecotoxicological testing for the marine Environment. Ed. by G. Persoone, E. Jaspers, & C. Claus. Lab. Biol. Res. Aquat. Pollut., State Univ. Ghent, Ghent,1, 307–339.
Thain, J. E. & Watts, J., 1984. The use of a bioassay to measure changes in water quality associated with a bloom ofGyrodinium aureolum (Hulbert). — C. M./ICES,D3 1–14.
Waterman, A. J., 1937. Effects of salts of heavy metals on the development of the sea urchin,Arbacia punctulata. — Biol. Bull. mar. biol. Lab., Woods Hole73 401–420.
Weichart, G., 1975. Untersuchungen über die Fe-Konzentration im Wasser der Deutschen Bucht im Zusammenhang mit dem Einbringen von Abwässern aus der Titandioxid-Produktion. — Dt. hydrogr. Z.28 49–61.
Woelke, C. E., 1972. Development of a receiving water quality bioassay criterion based on the 48-hour Pacific oyster (Crassostrea gigas) embryo. — Tech. Rep. Wash. Dep. Fish.9 1–93.
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Klöckner, K., Rosenthal, H. & Willführ, J. Invertebrate bioassays with North Sea water samples. I. Structural effects on embryos and larvae of serpulids, oysters and sea urchins. Helgolander Meeresunters 39, 1–19 (1985). https://doi.org/10.1007/BF01997515
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DOI: https://doi.org/10.1007/BF01997515