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Flagellar regeneration in the scaly green flagellateTetraselmis striata (Prasinophyceae): regeneration kinetics and effect of inhibitors
Helgoländer Meeresuntersuchungen volume 41, pages 149–164 (1987)
Abstract
Flagellar regeneration after experimental amputation was studied in synchronized axenic cultures of the scaly green flagellateTetraselmis striata (Prasinophyceae). After removal of flagella by mechanical shearing, 95% of the cells regrow all four flagella (incl. the scaly covering) to nearly full length with a linear velocity of 50 nm/min under standard conditions. Flagellar regeneration is independent of photosynthesis (no effect of DCMU; the same regeneration rate in the light or in the dark), but depends on de novo protein synthesis: cycloheximide at a low concentration (0.35 μM) blocks flagellar regeneration reversibly. No pool of flagellar precursors appears to be present throughout the flagellated phase of the cell cycle. A transient pool of flagellar precursors, sufficient to generate 2.5 μm of flagellar length, however, develops during flagellar regeneration. Tunicamycin (2 μg/ml) inhibits flagellar regeneration only after a second flagellar amputation, when flagella reach only one third the length of the control. Flagellar regeneration inT. striata differs considerably from that ofChlamydomonas reinhardtii and represents an excellent model system for the study of synchronous Golgi apparatus (GA) activation, and transport and exocytosis of GA-derived macromolecules (scales).
Literature Cited
Auclair, W. & Siegel, B. W., 1966. Cilia regeneration in the sea urchin embryo: evidence for a pool of ciliary proteins. —Science, N. Y.154, 913–915.
Baker, E. J., Schloss, J. A. & Rosenbaum, J. L., 1984. Rapid changes in tubulin RNA synthesis and stability induced by deflagellation inChlamydomonas. —J. Cell Biol.99, 2074–2081.
Bloodgood, R. A., 1982. Dynamic properties of the flagellar surface. —Symp. Soc. exp. Biol.35, 353–380.
Brunke, K. J., Young, E. E., Buchbinder, B. U. & Weeks, D. P., 1982. Coordinate regulation of the four tubulin genes ofChlamydomonas reinhardi. —Nucleic Acids Res.10, 1295–1310.
Ceriotti, G., 1952. Microchemical determination of DNA. —J. biol. Chem.198, 297–303.
Coggin, S. J. & Kochert, G., 1986. Flagellar development and regeneration inVolvox carteri (Chlorophyta). —J. Phycol.22, 370–381.
Fulton, C. & Kowit, J., 1975. Programmed synthesis of flagellar tubulin during cell differentiation inNaegleria. —Ann. N. Y. Acad. Sci.253, 318–332.
Geetha-Habib, M. & Bouck, G. B., 1982. Synthesis and mobilization of flagellar glycoproteins during regeneration inEuglena. —J. Cell Biol.93, 432–441.
Guttman, S. D. & Gorovsky, M. A., 1979. Cilia regeneration in starvedTetrahymena: an inducible system for studying gene expression and organelle biogenesis. —Cell17, 307–317.
Hoshaw, R. W. & Rosowski, J. R., 1973. Methods for microscopic algae. In: Handbook of phycological methods. Culture methods and growth measurements. Ed. by J. R. Stein. Cambridge University Press, Cambridge, 53–67.
Huang, B. P.-H., 1986.Chlamydomonas reinhardtii: a model system for the genetic analysis of flagellar structure and motility. —Int. Rev. Cytol.99, 181–215.
Huber, M. E., Wright, W. G. & Lewin, R. A., 1986. Divalent cations and flagellar autotomy inChlamydomonas reinhardtii (Volvocales, Chlorophyta). —Phycologia25, 408–411.
Lefebvre, P. A., Nordstrom, S. A., Moulder, J. E. & Rosenbaum, J. L., 1978. Flagellar elongation and shortening inChlamydomonas. IV. Effects of flagellar detachment, regeneration, and resorption on the induction of flagellar protein synthesis. —J. Cell Biol.78, 8–27
Lefebvre, P. A., Silflow, C. D., Wieben, E. D. & Rosenbaum, J. L., 1980. Increased levels of mRNAs for tubulin and other flagellar proteins after amputation or shortening ofChlamydomonas flagella. —Cell 20, 469–477.
Lewin, R. A., 1953. Studies on the flagella of algae. II. Formation of flagella byChlamydomonas in light and darkness. —Ann. N. Y. Acad. Sci.56, 1091–1093.
Lewin, R. A. & Lee, K. W., 1985. Autotomy of algal flagella: electron microscope studies ofChlamydomonas (Chlorophyceae) and Tetraselmis (Prasinophyceae). —Phycologia24, 311–316.
Lewin, R. A., Lee, T. H. & Fang, L. S., 1982. Effects of various agents on flagellar activity, flagellar autotomy and cell viability in four species ofChlamydomonas (Chlorophyta: Volvocales). —Symp. Soc. exp. Biol.35, 421–437.
L'Hernault, S. W. & Rosenbaum, J. L., 1983.Chlamydomonas α-tubulin is posttranslationally modified in the flagella during flagellar assembly —J. Cell Biol.97, 258–263.
Lilley, R. Mc. C., Fitzgerald, M. P., Rienits, K. G. & Walker, D. A., 1975. Criteria of intactness and the photosynthetic activity of spinach chloroplast preparations. —New Phytol.75, 1–10.
McFadden, G. I. & Wetherbee, R., 1985. Flagellar regeneration and associated scale deposition inPyramimonas gelidicola (Prasinophyceae, Chlorophyta). —Protoplasma128 31–37.
McFadden, G. I. & Melkonian, M., 1986a. Golgi apparatus activity and membrane flow during scale biogenesis in the green flagellateScherffelia dubia (Prasinophyceae). I.: Flagellar regeneration. —Protoplasma130, 186–198.
McFadden, G. I. & Melkonian, M., 1986b. Use of Hepes buffer for microalgal culture media and fixation for electron microscopy. —Phycologia25, 551–557.
McFadden, G. I., Schulze, D., Surek, B., Salisbury, J. L. & Melkonian, M., 1987. Basal body reorientation mediated by a Ca2+-modulated contractile protein. —J. Cell Biol. (In press.)
McLachlan, J., 1973. Growth media-marine. In. Handbook of phycological methods. Culture methods and growth measurements. Ed. by J. R. Stein. Cambridge University Press, Cambridge, 25–51.
Melkonian, M., 1982. Effect of divalent cations on flagellar scales in the green flagellateTetraselmis cordiformis. —Protoplasma111, 221–233.
Melkonian, M., 1987. Prasinophyceae. In: Handbook of protoctists. Ed. by L. Margulis, J. Corliss, M. Melkonian, D. J. Chapman. Jones & Bartlett, Boston (in press).
Melkonian, M., Preisig, H. R. & Lechtreck, F., 1985a.Scourfieldia, a most unusual green flagellate. Second Int. Phycol. Congr., Copenhagen. Abstr. 107.
Melkonian, M., Reize, I. B. & McFadden, G. I., 1985b. Flagellar scales in the green flagellateTetraselmis striata: isolation, characterization and biogenesis. —Eur. J. Cell Biol.36, 44.
Melkonian, M., McFadden, G. I., Reize, I. B. & Becker, D., 1986. Secretion of organic sales in green algae: secretory proteins are transported through the Golgi apparatus by cisternal progression. —Ber. dt. bot. Ges.99, 263–280.
Melkonian, M., Reize, I. B. & Preisig, H. R., 1987a. Maturation of a flagellum/basal body requires more than one cell cycle in algal flagellates: studies onNephroselmis olivacea (Prasinophyceae). In: Molecular and cellular aspects of algal development. Ed. by W. Wiessner, D. G. Robinson, R. C. Starr. Springer, Berlin (in press).
Melkonian, M., Becker, D., McFadden, G. I. & Reize, I. B., 1987b. Experimental analysis of membrane traffic during secretion of scales in green algae. In: Cell free analysis of membrane traffic. Ed. by J. Morre. Liss, New York (in press).
Moestrup, Ø., 1982. Flagellar structure in algae: a review with new observations particularly on the Chrysophyceae, Phaeophyceae (Fucophyceae), Euglenophyceae andReckertia. —Phycologia21, 427–528.
Nelsen, E. M., 1975. Regulation of tubulin during ciliary regeneration in non-growingTetrahymena. —Exp. Cell Res.94, 152–153.
Quader, H. & Glas, R., 1984. Geißelregeneration beiChlamydomonas reinhardtii. —Biol. unserer Zeit14, 125–127.
Randall, J., 1969. The flagellar apparatus as a model organelle for the study of growth and morphopoiesis. —Proc. R. Soc. (Ser. B)173, 31–62.
Remillard, S. P. & Witman, G. B., 1982. Synthesis, transport, and utilization of specific flagellar proteins during flagellar regeneration inChlamydomonas. —J. Cell Biol.93, 615–631.
Ricketts, T. R., 1974. Cultural requirements of the Prasinophyceae. —Nova Hedwigia25, 683–690.
Ricketts, T. R., 1979. The induction of synchronous cell division inPlatymonas striata Butcher (Prasinophyceae). —Br. phycol. J.14, 219–223.
Rosenbaum, J. L. & Child, F. M., 1967. Flagellar regeneration in protozoan flagellates. —J. Cell Biol.34, 345–364.
Rosenbaum, J. L., Moulder, J. E. & Ringo, D. L., 1969. Flagellar elongation and shortening inChlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins. —J. Cell Biol.41, 600–619.
Schloss, J. A., Silflow, C. D. & Rosenbaum, J. L., 1984. mRNA abundance changes during flagellar regeneration inChlamydomonas reinhardtii. —Mol. cell. Biol.4, 424–434.
Surek, B. & Melkonian, M., 1980. The filose amoebaVampyrellidium perforans nov. sp. (Vampyrellidae, Aconchulinida): axenic culture, feeding behavior and host range specificity. —Arch. Protistenk.123, 166–191.
Williams, B. D., Mitchell, D. R. & Rosenbaum, J. L., 1986. Molecular cloning and expression of flagellar radial spoke and dynein genes ofChlamydomonas. —J. Cell Biol.103, 1–11.
Witman, G. B., Carlson, K., Berliner, J. & Rosenbaum, J. L., 1972.Chlamydomonas flagella I. Isolation and electrophoretic analysis of microtubules, matrix, membranes and mastigonemes. —J. Cell Biol.54, 507–539.
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Reize, I.B., Melkonian, M. Flagellar regeneration in the scaly green flagellateTetraselmis striata (Prasinophyceae): regeneration kinetics and effect of inhibitors. Helgolander Meeresunters 41, 149–164 (1987). https://doi.org/10.1007/BF02364697
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DOI: https://doi.org/10.1007/BF02364697