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scDNA-DNA hybridization studies in Pacific and Caribbean isolates ofDictyosphaeria cavernosa (Chlorophyta) indicate a long divergence

Helgoländer Meeresuntersuchungen volume 41, pages 377–383 (1987)Cite this article

Abstract

Genomic size and complexity were calculated for the pantropical chlorophyte,Dictyosphaeria cavernosa (Cladophorales). Genome characterization of the Hawaiian material by means of DNA renaturation studies showed highly repetitive (31.3%), middle repetitive (42.7%), and single-copy (25.8%) fractions; and an estimated haploid genome size of 1.79 pg DNA. A G:C content of 43% was calculated from a melting curve of DNA. Pacific and Caribbean isolates of this species were compared using single-copy nuclear DNA-DNA hybridization. Results show a relatively low thermal stability of the hybridized DNA (Δ Tm(e)=10°C) which suggests that these Pacific and Caribbean lineages may have been separated for up to 55 Ma.

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Author notes

  1. J. L. Olsen

    Present address: Department of Botany, LSB-2017, University of California, 94720, Berkeley, CA, USA

Authors and Affiliations

  1. Department of Marine Biology, Biology Centre, University of Groningen, P. O. Box 14, NL-9750 AA, Haren (Gn), The Netherlands

    J. L. Olsen, W. T. Stam, P. V. M. Bot & C. van den Hoek

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  1. J. L. Olsen
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  2. W. T. Stam
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  3. P. V. M. Bot
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  4. C. van den Hoek
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Olsen, J.L., Stam, W.T., Bot, P.V.M. et al. scDNA-DNA hybridization studies in Pacific and Caribbean isolates ofDictyosphaeria cavernosa (Chlorophyta) indicate a long divergence. Helgolander Meeresunters 41, 377–383 (1987). https://doi.org/10.1007/BF02366200

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Helgoland Marine Research

ISSN: 1438-3888

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