- Published:
Growth patterns, chemical composition and oxygen consumption in early juvenileHyas araneus (Decapoda: Majidae) reared in the laboratory
Helgoländer Meeresuntersuchungen volume 46, pages 9–28 (1992)
Abstract
Early (instar I and II) juveniles of the spider crabHyas araneus were reared under constant conditions (12 °C, 32‰S) in the laboratory, and their growth, biochemical composition, and respiration were studied. Every second day, dry weight (W), ash-free dry weight (AFW), and contents of ash, organic and inorganic carbon (C), nitrogen (N), hydrogen (H), protein, chitin, lipid, and carbohydrates were measured, as well as oxygen consumption. Changes in the absolute amounts of W. AFW, and C, N, and H during the moulting cycle are described with various regression equations as functions of age within a given instar. These patterns of growth differ in part from those that have been observed during previous studies in larval stages of the same and some other decapod species, possibly indicating different growth strategies in larvae and juveniles. There were clear periodic changes in ash (% of W) and inorganic C (as % of total C), with initially very low and then steeply increasing values in postmoult, a maximum in intermoult, and decreasing figures during the premoult phase of each moulting cycle. Similar patterns were observed in the chitin fraction, reaching a maximum of 16% of W (31% of AFW). Ash, inorganic C, and chitin represent the major components of the exoskeleton and hence, changes in their amounts are associated with the formation and loss of cuticle material. Consequently, a high percentage of mineral matter was lost with the exuvia (76% of the late premoult [LPM] ash content, 74% of inorganic C), but relatively small fractions of LPM organic matter (15% of AFW, 11% of organic C, 5–6% of N and H). These cyclic changes in the cuticle caused an inverse pattern of variation in the percentage values (% of W) of AFW, organic C, N, H, and biochemical constituents other than chitin. When these measures of living biomass were related to, exclusively, the organic body fraction (AFM), much less variation was found during individual moulting cycles, with values of about 43–52% in organic C, 9–10% in N, 6–9% H, 31–49% of AFW in protein, 3–10% in lipid, and <1% in carbohydrates. All these constituents showed, on the average, a decreasing tendency during the first two crab instars, whereas N remained fairly constant. It cannot be explained at present, what other elements and biochemical compounds, respectively, might replace these decreasing components of AFW. Decreasing tendencies during juvenile growth were observed also in the organic C/N and in the lipid/protein weight ratios, both indicating that the proportion of lipid decreased at a higher rate than that of protein. Changes were observed also in the composition of inorganic matter, with significantly lower inorganic C in early postmoult (2–4% of ash) than in later stages of the moult cycle (about 9%). This reflected probably an increase in the degree of calcification, i.e. in the calcium carbonate content of the exoskeleton. As a fraction of total C, inorganic C reached maximum values of 17 and 20% in the crab I and II instars, respectively. The energy content of juvenile spider crabs was estimated independently from organic C and biochemical constituents, with a significant correlation between these values. However, the former estimates of energy were, on the average, significantly lower than the latter (slope of the regression ≠1). Since organic C should be a reliable integrator of organic substances, but the sum of protein, lipid, chitin, and carbohydrates amounted to only 60–91% of AFW, it is concluded that the observed discrepancy between these two estimates of energy was caused by energy from biochemical constituents that had not been determined in our analyses. Thus, energy values obtained from these biochemical fractions alone may underestimate the actual amount of organic matter and energy. Respiration per individual in juvenile spider crabs was higher than that in larval stages of the same species (previous studies), but their W-specific values of oxygen consumption (QO2) were lower than in conspecific larvae (0.6–2μg O2·[mg W]−1). QO2 showed a consistent periodic pattern in relation to the moult cycle: maximum values in early postmoult, followed by a rapid decrease, and constant values in the intermoult and premoult phases. This variation is interpreted as an effect mainly of cyclic changes in the amounts of cuticle materials which are metabolically inactive. From growth and respiration values (both expressed in units of organic C), net growth efficiency, K2, values may be calculated. In contrast to previous findings in larval stages, K2 showed an increasing trend during growth of the first two juvenile instars ofH. araneus.
Literature Cited
Anger, K., 1984. Gain and loss of particulate organic and inorganic matter in larval and juvenile spider crabs (Hyas araneus) during growth and exuviation. — Helgoländer Meeresunters.38, 107–122.
Anger, K., 1990. Modelling developmental changes in the carbon and nitrogen budgets of larval brachyuran crabs. — Helgoländer Meeresunters.44, 53–80.
Anger, K., 1991. Developmental changes in the bioenergetics of decapod larvae. — Men. Qd Mus.31, 289–308.
Anger, K. & Harms, J., 1990. Elemental (CHN) and proximate biochemical composition of decapod crustacean larvae. — Comp. Biochem. Physiol.97B, 69–80.
Anger, K. & Hirche, H. J., 1990. Nucleic acids and growth of larval and early juvenile spider crabHyas araneus. — Mar. Biol.105, 403–411.
Anger, K., Harms, J., Püschel, C. & Seeger, B., 1989. Physiological and biochemical changes during the larval development of a brachyuran crab reared under constant conditions in the laboratory. —Helgoländer Meeresunters.43, 225–244.
Anger, K., Laasch, N., Püschel, C. & Schorn, F., 1983. Changes in biomass and chemical composition of spider crab (Hyas araneus) larvae reared in the laboratory. — Mar. Ecol. Prog. Ser.12, 91–101.
Bulnheim, H.-P., 1974. Respiratory metabolism ofIdotea balthica (Crustacea, Isopoda) in relation to environmental variables, acclimation processes and moulting. — Helgoländer wiss. Meeresunters.26, 464–480.
Drach, P. & Lafon, M., 1942. Études biochimiques sur le squelette tégumentaire des décapodes brachyoures (variation au cours du cycle d’intermue). — Archs Zool. exp. gén.82, 100–118.
Gnaiger, E., 1983. Calculation of energetic and biochemical equivalents of respiratory oxygen consumption. In: Polarographic oxygen sensors. Ed. by E. Gnaiger & H. Forstner. Springer, Berlin, 337–345.
Gwinn, J. F. & Stevenson, J. R., 1973. Role of acetylglucosamine in chitin synthesis in crayfish. I. Correlation of14C-acetylglucosamine incorporation with stages of the molting cycle. — Comp. Biochem. Physiol.45B, 769–776
Harms, J., Anger, K., Klaus, S. & Seeger, B., 1991. Nutritional effects on ingestion rate, digestive enzyme activity, growth, and biochemical composition ofHyas araneus L. (Decapoda: Majidae) larvae. — J. exp. mar. Biol. Ecol.145, 233–265.
Heath, J. R. & Barnes, H., 1970. Some changes in biochemical composition with season and during the moulting cycle of the common shore crab,Carcinus maenas (L.). — J. exp. mar. Biol. Ecol.5, 199–233.
Hirota, J. & Szyper, J. P., 1975. Separation of total particulate carbon into inorganic and organic components. — Limnol. Oceanogr.20, 896–900.
Holland, D. L. & Gabbott, P. A., 1971. A micro-analytical scheme for the determination of protein, carbonhydrate, lipid and RNA levels in marine invertebrate larvae. — J. mar. biol. Ass. U.K.51, 659–668.
Hornung, D. E. & Stevenson, J. R., 1971. Changes in the rate of chitin synthesis during the crayfish molting cycle. — Comp. Biochem. Physiol.40B, 341–346.
Kunisch, M. & Anger, K., 1984. Variation in development and growth rates of larval and juvenile spider crabsHyas araneus reared in the laboratory. — Mar. Ecol. Prog. Ser.15, 293–301.
Lowry, D. H., Rosenberg, N. J., Farr, A. L. & Randall, R. J., 1951. Protein measurement with the folin phenol reagent. — J. biol. Chem.193, 265–275.
McMahon, B. R. & Wilkens, J. L., 1983. Ventilation, perfusion, and oxygen uptake. In: The biology of Crustacea. Ed. by L. H. Mantel. Acad. Press, New York,5, 289–372.
Olst, J. C. van, Carlberg, J. M. & Hughes, J. T., 1980. Aquaculture. In: The biology and management of lobsters. Ed. by J. S. Cobb & B. F. Phillips. Acad. Press, New York,2, 333–384.
Raymont, J. E. G., Austin, J. & Linford, E., 1964. Biochemical studies on marine zooplankton. I. The biochemical composition ofNeomysis integer. — J. Cons. perm. int. Explor. Mer28, 354–363.
Renaud, L., 1949. Le cycle des réserves organiques chez les crustacés Décapodes. — Annls Inst. océanogr. Monaco24, 259–357.
Sachs, L., 1984. Angewandte Statistik. Springer, Berlin, 552 pp.
Salonen, K., Sarvala, J., Hakala, I. & Viljanen, M.-L., 1976. The relation of energy and organic carbon in aquatic invertebrates. — Limnol. Oceanogr.21, 724–730.
Speck, U. & Urich, K., 1971. Quantitative Bedeutung der Reservestoffe für Chitinsynthese, Energiestoffwechsel und osmotische Vorgänge während der Häutung des FlußkrebsesOrconectes limosus. — Z. vergl. Physiol.71, 286–294.
Speck, U. & Urich, K., 1972. Resorption des alten Panzers vor der Häutung bei dem FlußkrebsOrconectes limosus. Schicksal des freigesetzten N-Acetylglucosamins. — J. comp. Physiol.78, 210–220.
Speck, U., Urich, K. & Hahmann, R., 1972. Der Stoffbestand des FlußkrebsesOrconectes limosus. Jahreszyklus und Organverteilung. — J. comp. Physiol.77, 287–305.
Spidler-Barth, M., 1976. Changes in the chemical composition of the common shore crab,Carcinus maenas, during the molting cycle. — J. comp. Physiol.105, 197–205.
Winberg, G. G., 1971. Methods for the estimation of production of aquatic animals. Acad. Press, London, 175 pp.
Zöllner, N. & Kirsch, K., 1962. Über die quantitative Bestimmung von Lipoiden (Mikromethode) mittels der vielen natürlichen Lipoiden (allen bekannten Plasmalipoiden) gemeinsamen Sulfophosphovanillin-Reaktion. — Z. ges. exp. Med.135, 545–561.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Anger, K., Harms, J., Christiansen, M.E. et al. Growth patterns, chemical composition and oxygen consumption in early juvenileHyas araneus (Decapoda: Majidae) reared in the laboratory. Helgolander Meeresunters 46, 9–28 (1992). https://doi.org/10.1007/BF02366209
Issue Date:
DOI: https://doi.org/10.1007/BF02366209