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Do test cells secrete a hatching enzyme inAscidiella aspersa (Tunicata, Ascidiacea)?

Helgoländer Meeresuntersuchungen volume 49, pages 295–301 (1995)Cite this article

Abstract

The hatching enzyme ofAscidiella aspersa has been characterized as a trypsin-like enzyme. It dissolves the main part of the chorion and renders hatching possible at the end of embryogenesis. In contrast to Knaben's statement, this enzyme is secreted by the embryo itself and not by the test cells.

Literature Cited

  • Acyagi, T., Takeuchi, T., Matsusaki, A., Kawamura, K., Kondo, S., Hamada, H., Maeda, K. & Umezawa, H., 1969. Leupeptins, new protease inhibitors from actinomycetes. — J. Antibiot.22, 283–286.

    Google Scholar 

  • Berrill, N. J., 1929. Studies in tunicate development. I. General physiology of the development of simple ascidians. — Phil. Trans. R. Soc. (Ser. B)218, 37–78.

    Google Scholar 

  • Caggegi, S., Flugy, A., Puccia, E. & Reverberi, G., 1974. On the hatching of ascidian larvae — Atti Accad. naz. Lincei Rc. (Ser. 9)56, 803–807.

    Google Scholar 

  • Cavey, M. J., 1976. Ornamentation of the larval ascidian tunic by test cells. — J. Ultrastruct. Res.55, 297–298.

    Google Scholar 

  • Cloney, R. A. & Cavey, M. J., 1982. Ascidian larval tunic: extraembryonic structures influence morphogenesis. — Cell Tissue Res.222, 547–562.

    Article  CAS  PubMed  Google Scholar 

  • Denucé, J. M., 1975. Proteolytic activity inCiona intestinalis associated with hatching. — Archs int. Physiol. Biochim.83, 958–959.

    Google Scholar 

  • Gordon, J., Levin, B. & Whitehead, T. P., 1952. Rapid screening test for trypsin in duodenal juice. In: McAllister, R. A., Enzymes and the determination of enzyme activity. Ed. by R. A. McAllister. Butterworth, London, 66 pp.

    Google Scholar 

  • Hoshi, M. & Numakunai, T., 1981. Hatching enzyme of the solitary ascidianHalocynthia roretzi. —Acta Embryol. Morph. exp.2, 163–169.

    CAS  Google Scholar 

  • Kessel, R. G. & Kemp, N. E., 1962. An electron microscopy study on the oocyte, test cells and follicular envelope of the tunicateMolgula manhattensis. — J. Ultrastruct. Res.6, 57–76.

    CAS  PubMed  Google Scholar 

  • Knaben, N., 1936. Über die Entwicklung und die Funktion der Testazellen beiCorella parallelogramma Mull. — Bergens Mus. Årb. (Naturv. R.)1, 34.

    Google Scholar 

  • Lübbering, B., Niermann-Kerkenberg, E. & Hofmann, D. K., 1992. Test cells ofAscidiella aspersa: adhesion and migration behaviour during embryogenesis. — Invert. Reprod. Dev.21, 241–252.

    Google Scholar 

  • Luft, J. H., 1961. Improvements in epoxy resin embedding methods. — J. biophys. biochem. Cytol.9, 409–414.

    CAS  PubMed  Google Scholar 

  • Mancuso, V., 1965. An electron microscope study of the test cells and follicle cells ofCiona intestinalis during oogenesis. — Acta Embryol. Morph. exp.8, 239–266.

    CAS  Google Scholar 

  • Niermann-Kerkenberg, E. & Hofmann, D. K., 1989. Fertilisation and normal development inAscidiella aspersa studied with Nomarski optics. — Helgoländer Meeresunters.43, 245–258.

    Google Scholar 

  • Pérèz, J. M., 1954. Considération sur le fonctionnement ovarien chezCiona intestinalis. — Archs Anat. microsc. Morph. exp.43, 58–78.

    Google Scholar 

  • Reverberi, G., 1978. Observations on the ultrastructure of the test cells ofMolgula impura. — Acta Embryol. exp.2, 229–245.

    Google Scholar 

  • Reynolds, E. S., 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. — J. Cell Biol.17, 208–212.

    Article  CAS  PubMed  Google Scholar 

  • Umezawa, H., 1976a. Bestatin, an inhibitor of aminopeptidase B produced by micromycetes. — J. Antibiot.29, 97–99.

    CAS  PubMed  Google Scholar 

  • Umezawa, H., 1976b. Structures and activities of protease inhibitors of microbial origin. — Methods Enzym.45, 678–695.

    CAS  Google Scholar 

  • Umezawa, H., 1977. Protease inhibitors produced by microorganisms. — Acta biol. med. germ.36, 1899–1915.

    CAS  PubMed  Google Scholar 

  • Vonis Bidlingmeyer, U. de, 1972. Identity of tryptic and α-chymotryptic reactive sites on soy bean trypsin inhibitor. — Biochemistry11, 3303–3310.

    PubMed  Google Scholar 

  • Werle, E., 1964. Über einen Hemmstoff für Kallikrein und Trypsin aus der Rinderlunge. — Hoppe-Seyler's Z. physiol. Chem.338, 228.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institut de Zoologie, Laboratoire de Biologie générale et de Morphologie ultrastructurale, Université de Liège, 22 Quai Van Beneden, B-4020, Liège, Belgium

    B. Lübbering

  2. Lehrstuhl für Spezielle Zoologie & Parasitologie, AG Entwicklungsphysiologie der Tiere, Ruhr-Universität Bochum, D-44789, Bochum, Germany

    D. K. Hofmann

Authors
  1. B. Lübbering
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  2. D. K. Hofmann
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Lübbering, B., Hofmann, D.K. Do test cells secrete a hatching enzyme inAscidiella aspersa (Tunicata, Ascidiacea)?. Helgolander Meeresunters 49, 295–301 (1995). https://doi.org/10.1007/BF02368357

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Helgoland Marine Research

ISSN: 1438-3888

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