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  • North Sea Research: Marine Ecophysiology, Parasite-Host Relationships
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Acidification of the gill cells of the shore crabCarcinus mediterraneus: Its physiological significance

Abstract

In a preparation of isolated gills of the shore crabCarcinus mediterraneus perfused with dilute sea water (pH 8.1, 200 mM Na+) which was identical to the bathing solution of the gill, acidification of the collected perfusate was observed. Acidification was not affected by 10−4 M EIPA (5-[N-ethyl-N-isopropyl]amiloride), a strong inhibitor of Na+/H+ exchange. However, in the presence of 10−4 M acetazolamide, acidification was greatly blocked. The significant decrease of the acid load of the perfusate is considered to be a result of inhibition of the branchial intracellular carbonic anhydrase catalyzing the formation of H+ ions.

Literature Cited

  • Ahearn, G. A. & Clay, L. P., 1989. Kinetic analysis of electrogenic 2Na+−1H+ antiport in crustacean hepatopancreas. — Am. J. Physiol.257, R484-R493.

    CAS  PubMed  Google Scholar 

  • Böttcher, K., Siebers, D. & Becker, W., 1990. Carbonic anhydrase in branchial tissues of osmoregulating shore crabsCarcinus maenas. — J. exp. Zool.255, 251–256.

    Article  Google Scholar 

  • Burnett, L. E. & Towle, D. W., 1990. Sodium ion uptake by perfused gills of blue crabCallinectes sapidus. — J. exp. Biol.149, 293–305.

    CAS  Google Scholar 

  • Cameron, J. M. & Batterton, C. V., 1976. Antennal gland function in the freshwater blue crabCallinectes sapidus. — J. comp. Physiol.133, 219–225.

    Google Scholar 

  • Henry, R. P., 1987. Membrane-associated carbonic anhydrase in the gills of the blue crabCallinectes sapidus. — J. exp. Zool.245, 1–8.

    Google Scholar 

  • Kleyman, T. R. & Cragoe, E. J., 1988. Amiloride and its analogs as tools in the study of ion transport. —J. Membrane Biol.105, 1–21.

    Article  CAS  Google Scholar 

  • Krippeit-Drews, P., Drews, G. & Graszynsky, K., 1989. Effects of ion substitution on the transepithelial potential difference of the gills of the fiddler crabUca tangeri: evidence for a H+-pump in the apical membrane. — J. comp. Physiol. (B)159, 43–49.

    CAS  Google Scholar 

  • Lin, H. & Randall, D., 1991. Evidence for the presence of an electrogenic proton pump in the trout gill epithelium. — J. exp. Biol.161, 119–134.

    Google Scholar 

  • Lucu, Č., 1993. Ion transport in the gill epithelium of aquatic crustacea. — J. exp. Zool.265, 378–386.

    CAS  Google Scholar 

  • Lucu, Č. & Siebers, D., 1986. Amiloride sensitive sodium flux and potentials in perfusedCarcinus gill preparation. — J. exp. Biol.122, 25–35.

    Google Scholar 

  • Lucu, Č., Devescovi, M. & Siebers, D., 1989. Do amiloride and ouabain affect ammonia fluxes in perfusedCarcinus gill epithelium? — J. exp. Zool.249, 1–5.

    Article  CAS  PubMed  Google Scholar 

  • Perry, S. F., Davie, P. S., Daxboeck, C. & Randall, D., 1982. A comparison of CO2 excretion in a spontaneously ventilating blood-perfused trout preparation and saline-perfused gill preparations. — J. exp. Biol.101, 47–60.

    Google Scholar 

  • Shetlar, R. E. & Towle, D. W., 1989. Electrogenic sodium-proton exchange in membrane vesicles from crab (Carcinus maenas) gill. — Am. J. Physiol.257, R924-R931.

    CAS  PubMed  Google Scholar 

  • Swenson, E. R. & Maren, T. H., 1987. Roles of gill and red cell carbonic anhydrase in elasmobranch HCO3 and CO2 excretion. — Am. J. Physiol.253, R450-R458.

    CAS  PubMed  Google Scholar 

  • Wheatly, M. G. & Henry, R. P., 1992. Extracellular and intracellular acid-base regulation in crustaceans. — J. exp. Zool.263, 127–142.

    Article  CAS  Google Scholar 

  • Wright, P. A., Randall, D. J. & Parry, S. F., 1989. Fish gill water boundary layer, a site of linkage between carbon dioxide and ammonia excretion. — J. comp. Physiol. (B)158, 627–635.

    Google Scholar 

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Lucu, Č., Siebers, D. Acidification of the gill cells of the shore crabCarcinus mediterraneus: Its physiological significance. Helgolander Meeresunters 49, 709–713 (1995). https://doi.org/10.1007/BF02368394

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