- Modern Techniques in Marine Ecology
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Pheromones in marine algae: A technical approach
Helgoländer Meeresuntersuchungen volume 49, pages 797–804 (1995)
Abstract
It is now well known that many marine organisms use low-molecular volatile substances as signals, in order to coordinate activities between different individuals. The study of such pheromones requires the isolation and enrichment of the secretions from undisturbed living cells or organisms over extended periods of time. The Grob-Hersch extraction device, which we describe here, avoids adverse factors for the biological materials such as strong water currents, rising gas bubbles or chemical solvents. Furthermore, the formation of sea-water spray is greatly reduced. The application of this technique for the isolation of pheromones of marine algae and animals is described.
Literature Cited
Blanchard, D. C. & Syzdek, L. D., 1975. Electrostatic collection of jet and film drops. — Limnol. Oceanogr.20, 762–774.
Boland, W., Ney, P., Jaenicke, L. & Gassmann, G., 1984. A “closed-loop-stripping” technique as a versatile tool for metabolic studies of volatiles. In: Analysis of volatiles. Ed. by P. Scheuer. De Gruyter, Berlin, 371–380.
Boland, W., Jaenicke, L. & Müller, D. G., 1985. Viridiene—the male gamete attractant of the brown algaSyringoderma phinneyi—has 3R, 4S-configuration. — Naturwissenschaften72, 147–148.
Boland, W., Jaenicke, L., Müller, D. G. & Gassmann, G., 1987. Giffordene, 2Z, 4Z, 6E, 8Z-undecatetraene, is the odoriferous principle of the marine brown algaGiffordia mitchellae. —Experientia43, 466–467.
Cooney, D. O., Nagerl, A. & Hines, A. L., 1983. Solvent regeneration of activated carbon. — Wat. Res.17, 403–410.
Friant, S. L. & Suffet, I. H., 1979. Interactive effects of temperature, salt concentration, and pH on head space analysis for isolating volatile trace organics in aqueous environmental samples. —Analyt. Chem.51, 2167–2172.
Gassmann, G., 1973. Untersuchung zur Isolierung und Struktur von Metallproteiden ausClostridium pasteurianum. Diss., Univ. Tübingen, 136 pp.
Gassmann, G. & Glindemann, D., 1993. Phosphan (PH3) in der Biosphäre. — Angew. Chem.105, 749–751. (Angew. Chem. int. Ed.32, 761–763).
Gassmann, E. & Schorn, F., 1993. Phosphine from harbor surface sediments. — Naturwissenschaften80, 78–80.
Gilroy, D. & Mayne, J. E. O., 1962. The de-aeration of aqueous solutions. — J. appl. Chem.12, 382–384.
Grob, K., 1973. Organic substances in potable water and in its precursors. Pt I. Methods for their determination by gas-liquid chromatography. — J. Chromatogr.84, 255–273.
Grob, K. & Grob, G., 1974. Organic substances in potable water and in its precursors. Pt II. Application in the area of Zürich. — J. Chromatogr.90, 303–313.
Grob, K. & Zürcher, F., 1976. Stripping of trace organic substances from water: equipment and procedure. — J. Chromatogr.117, 285–294.
Grob, K., Grob, K. jr. & Grob, G., 1975. Organic substances in potable water and in its precursors. Pt III. The closed-loop stripping procedure compared with rapid liquid extraction. — J. Chromatogr.106, 299–315.
Jaenicke, L. & Seferiadis, K., 1975. Die Stereochemie von Fucoserraten, dem Gametenlockstoff der BraunalgeFucus serratus L. — Chem. Ber.108, 225–232.
Jaenicke, L., Müller, D. G. & Moore, R. E., 1974. Multifidene and Aucantene, C11 hydrocarbons in the male-attracting essential oil from the gynogametes ofCutleria multifida (Smith) Grev. (Phaeophyta). — J. Am. chem. Soc.96, 3324–3325.
Marner, F. J., Müller, B. & Jaenicke L., 1984. Lamoxirene. Structural proof of the spermatozoid releasing and attracting pheromone ofLaminariales. — Z. Naturf.39c, 689–691.
Müller, D. G., 1967. Ein leicht flüchtiges Gyno-Gamon der BraunalgeEctocarpus siliculosus. —Naturwissenschaften54, 496–497.
Müller, D. G., 1974. Sexual reproduction and isolation of the sex attractant inCutleria multifida (Smith) Grev. (Phaeophyta). — Biochem. Physiol. Pflanz.165, 212–215.
Müller, D. G., 1976. Quantitative evaluation of sexual chemotaxis in two marine brown algae. — Z. Pflanzenphysiol.80, 120–130.
Müller, D. G. & Gassmann, G., 1978. Identification of the sex attractant in the marine brown algaFucus vesiculosus. — Naturwissenschaften65, 389.
Müller, D. G. & Jaenicke, L., 1973. Fucoserraten, the female sex attractant ofFucus serratus L. (Phaeophyceae). — FEBS Lett.30 137–139.
Müller, D. G., Jaenicke, L., Donike, M., & Akintobi, T., 1971. Sex attractant in a brown alga: chemical structure. — Science, N. Y.171, 815–817.
Müller, D. G., Gassmann, G. & Lüning, K., 1979. Isolation of a spermatozoid-releasing and-attracting substance from female gametophytes ofLaminaria digitata. — Nature, Lond.279, 430–431.
Müller, D. G., Gassmann, G., Boland, W., Marner, F. & Jaenicke, L., 1981a.Dictyota dichotoma (Phaeophyceae): identification of the sperm attractant. — Science, N. Y.212 1040–1041.
Müller, D. G., Marner, F.J., Boland, W. Jaenicke, L. & Gassmann, G., 1981b. Identification of a volatile gamete secretion inSpermatochnus paradoxus — Naturwissenschaften67, 478–479.
Müller, D. G., Peters, A., Gassmann, G., Boland, W., Marner, F.-J. & Jaenicke, L., 1982. Identification of a sexual hormone and related substances in the marine brown algaDesmarestia. —Naturwissenschaften69, 290–292.
Müller, D. G., Clayton, M. N., Gassmann, G., Boland, W., Marner, F. J. & Jaenicke, L., 1984. The sperm attractant ofHormosira banksii (Phaeophyceae, Fucales), a seaweed common to Australia and New Zealand. — Experientia40, 211–212.
Müller, D. G., Clayton, M. N., Gassmann, G., Boland, W., Marner, F.-J., Schotten, T. & Jaenicke, L., 1985. Cystophorene and hormosirene, sperm attractants in Australian brown algae. —Naturwissenschaften72, 97–99.
Müller, D. G., Boland, W., Becker, U. & Wahl, T., 1988. Caudoxirene, the spermatozoid-releasing and attracting factor in the marine brown algaPerithalia caudata (Phaeophyceae, Sporochnales). — Biol. Chem. Hoppe-Seyler369, 655–659.
Wu, J. 1981. Evidence of sea spray produced by bursting bubbles. — Science, N. Y.212, 324–326.
Zeeck, E., Hardege, J., Bartels-Hardege, H. & Wesselmann, G., 1988. Sex pheromone in a marine polychaete: determination of the chemical structure. — J. exp. Zool.246, 285–292.
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Gassmann, G., Müller, D.G. & Fritz, P. Pheromones in marine algae: A technical approach. Helgolander Meeresunters 49, 797–804 (1995). https://doi.org/10.1007/BF02368402
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DOI: https://doi.org/10.1007/BF02368402