- Heavy-Metal Pollution
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Accumulation, loss and molecular distribution of cadmium inMytilus edulis
Helgoländer Meeresuntersuchungen volume 33, pages 68–78 (1980)
Abstract
InMytilus edulis, accumulation and loss of Cd were analyzed under experimental conditions. Cd uptake by the whole soft body is linear, increasing significantly with increasing Cd concentrations in the uptake medium. Until 100 µg Cd l−1, neither limitation of uptake nor any saturation process can be observed. Loss of Cd, measured after transfer of experimentally contaminated mussels to natural sea water, is exponential; biological half lives vary between 14 and 29 days. Gills are the primary sites of Cd uptake from the water, whereas in mid-gut gland, kidney, and mantle the uptake is retarded during the first few days. The mid-gut gland not only bears the main body load of Cd, but also shows the highest Cd concentrations. Gel chromatographic studies of mid-gut gland proteins reveal that Cd is eluated over the whole molecular weight range. Three metallothionein-like proteins with molecular weights of 6,600, 13,200, and 21,000 Dalton could be established. However, they cannot be taken as effective detoxification proteins, because more than 50% of the accumulated metal is bound to high molecular weight proteins.
Literature cited
Friberg, L., Piscator, H., Nordberg, G. F. & Kjellström, T., 1976. Cadmium in the environment. CRC-Press, Cleveland, Ohio, 248 pp.
George, S. G. & Coombs, T. L., 1977. The effects of chelating agents on the uptake and accumulation of cadmium byMytilus edulis. — Mar. Biol.39, 261–268.
Howard, A. G. & Nickless, G., 1977. Heavy metal complexation in polluted molluscs. I. Limpets (Patella vulgata andPatella intermedia). — Chem.- biol. Interact.16, 107–114.
Janssen, H. H. & Scholz, N., 1979. Uptake and cellular distribution of cadmium inMytilus edulis. — Mar. Biol.55, 133–141.
Kägi, J. H. R. & Vallee, B. L., 1960. Metallothionein: a cadmium and zinc containing protein from equine renal cortex. — J. biol. Chem.235, 3460–3465.
Karbe, L., 1972. Marine Hydroiden als Testorganismen zur Prüfung der Toxizität von Abwasserstoffen. Die Wirkungen von Schwermetallen auf Kolonien vonEirene viridula. — Mar. Biol.12, 316–328.
Noel-Lambot, F., 1976. Distribution of cadmium, zinc, and copper in the musselMytilus edulis. Existence of cadmium-binding proteins similar to metallothionein. — Experientia32, 324–326.
Noel-Lambot, F., 1979. Cadmium accumulation correlated with increase in metallothionein concentration in the limpetPatella caerulea. In: Animals and environmental fitness. Reprints of abstracts. Ed. by R. Gilles. Pergamon Press, Oxford, 83–84.
Noel-Lambot, F., Bouquegneau, J. M., Frankenne, F., & Disteche, A., 1978. Le role metallothioneines dans le stockage des métaux lourds chez les animaux marins. — Revue int. Océanogr. méd.44, 13–20.
Olafson, R. W. & Thompson, J. A. J., 1974. Isolation of heavy metal binding proteins from marine vertebrates. — Mar. Biol.28, 83–86.
Overnell, J., Davidson, J. A. & Coombs, T. L., 1977. A cadmium binding glycoprotein from the liver of the plaice(Pleuronectes platessa). — Biochem. Soc. Trans.5, 267–269.
Phillips, D. J. H., 1977. The use of biological indicator organisms to monitor trace metal pollution in marine and estuarine environments — a review. — Environ. Pollut.13, 281–317.
Probst, G. S., Bousquet, W. T. & Miya, T. S., 1977. Correlation of metallothionein concentrations with acute cadmium toxicity in the mouse. — Toxic. appl. Pharmac.39, 61–69.
Schulz-Baldes, M., 1974. Lead uptake from sea water and food, and lead loss in the common mussel,Mytilus edulis. — Mar. Biol.25, 177–193.
Squibb, K. S., Cousins, R. J., Silbon, B. L. & Levin, S., 1976. Liver and intestinal metallothionein: function in acute cadmium toxicity. — Exp. mol. Path.25, 163–171.
Tanaka, K., Sueda, K., Onosaka, S. & Okahara, K., 1975. Fate of109Cd-labelled metallothionein in rats. — Toxic. appl. Pharmac.33, 258–266.
Theede, H., Andersson, I. & Lehnberg, W., 1979a. Cadmium inMytilus edulis from German coastal waters. — Meeresforsch.27, 147–155.
Theede, H., Scholz, N. & Fischer, H., 1979b. Temperature and salinity effects on the acute toxity of cadmium toLaomedea loveni (Hydrozoa). — Mar. Ecol. Prog. Ser.1, 13–19.
Webb, M., 1975. Metallothionein and the toxicity of cadmium. In: Ecological toxicology research. Ed. by A. D. McIntyre & C. F. Mills. Plenum Press, New York, 177–186.
Wolf, P. de, 1975. Mercury content of mussels from West European coasts. — Mar. Pollut. Bull.6, 61–63.
Wright, D. A., 1977. The effect of calcium on cadmium uptake by the shore crabCarcinus maenas. — J. exp. Biol.67, 163–173.
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Scholz, N. Accumulation, loss and molecular distribution of cadmium inMytilus edulis . Helgolander Meeresunters 33, 68–78 (1980). https://doi.org/10.1007/BF02414736
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DOI: https://doi.org/10.1007/BF02414736