Skip to main content
  • Oil, Oil Dispersants And Related Substances
  • Published:

Determination and localization of oil components in living benthic organisms by fluorescence microscopy

Abstract

Microspectrofluorometric measurements are made to determine uptake and distribution of oil in marine organisms after exposure to crude oil. Equipment combining fluorescence microscopy with spectral analysis of the fluorescence emission is described. After contamination with oil, the intestine content ofLumbricillus lineatus, Nereis diversicolor andAnaitides mucosa shows a fluorescence emission at long wavelengths with a maximum at about 550 nm; this is in contrast to the fluorescence emission of these organisms without oil contamination. There is evidence that aromatic hydrocarbons are metabolized in the intestine of the worms studied.

Literature cited

  • Anderson, J. W., 1977. Responses to sublethal levels of petroleum hydrocarbons: Are they sensitive indicators and do they correlate with tissue contamination? In: Fate and effects of petroleum hydrocarbons in marine organisms and ecosystems. Ed. by D. A. Wolfe. Pergamon Press, Oxford, 95–114.

    Google Scholar 

  • Anderson, R. S., 1978. Developing an invertebrate model for chemical carcinogenesis: Metabolic activation of carcinogenesis. — Comp. Pathobiol.4, 11–24.

    CAS  Google Scholar 

  • Berlman, I. B., 1971. Handbook of fluorescence spectra of aromatic molecules. Acad. Press, New York, 379 pp.

    Google Scholar 

  • Ernst, W., 1979. Metabolic transformation of 1-(1-14C)naphthol in bioconcentration studies with the common musselMytilus edulis. — Veröff. Inst. Meeresforsch. Bremerhaven17, 233–240.

    CAS  Google Scholar 

  • Gibson, D. T., 1976. Initial reactions in the bacterial degradation of aromatic hydrocarbons. — Zbl. Bakt., ParasitKde. (Abt. 1., Reihe B)162, 157–168.

    CAS  Google Scholar 

  • Gordon, D. C. & Keizer, P. D., 1974. Estimation of petroleum hydrocarbons in sea water by fluorescence spectroscopy: Improved sampling and analytical methods. — Tech. Rep. Fish. Res. Bd Can.481, 1–29.

    Google Scholar 

  • Hodgins, H. O., Gronlund, W. D., Mighell, J. L., Hawkes, J. W. & Robish, P. A., 1977. Effect of crude oil on trout reproduction. In: Fate and effects of petroleum hydrocarbons in marine organisms and ecosystems. Ed. by D. A. Wolfe. Pergamon Press, Oxford, 143–150.

    Google Scholar 

  • Jerina, D. M., Yagi, H., Thakker, D. R., Karle, J. M., Mah, H. D., Boyd, D. R., Gadaginamath, G., Wood, A. W., Buening, M. et al., 1979. Stereoselective metabolic activation of polycyclic aromatic hydrocarbons. In: Pharmacology: international congress proceedings. Ed. by Y. Cohen. Pergamon Press, Oxford,7 (11), 53–62.

    Google Scholar 

  • Kohen, E., Kohen, C., Hirschberg, J. G., Wouters, A. & Thorell, B., 1978. Multisite topographic microspectrofluorometry of intercellular and exogeneous fluorochromes. — Photochem. Photobiol.27, 259.

    CAS  PubMed  Google Scholar 

  • Malins, D. C., 1977. Metabolism of aromatic hydrocarbons in marine organisms. — Ann. N. Y. Acad. Sci.298, 482–486.

    CAS  Google Scholar 

  • Schäfer-Ridder, M., 1979. Carcinogenese durch polycyclische aromatische Kohlenwasserstoffe. — Nachr. Chem. Tech.27, 4–6.

    Google Scholar 

  • Sims, P. & Grover, P. I., 1974. Epoxides in polycyclic aromatic hydrocarbon metabolism and carcinogenesis. — Adv. Cancer Res.20, 165–172.

    Article  CAS  PubMed  Google Scholar 

  • Varanasi, U. & Malins, D. C., 1977. Metabolism of petroleum hydrocarbons: Accumulation and biotransformation in marine organisms. In: Effects of petroleum on arctic and subarctic marine environments and organisms. Ed. by D. C. Malins. Acad. Press, New York1, 1–321.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zeeck, E. Determination and localization of oil components in living benthic organisms by fluorescence microscopy. Helgolander Meeresunters 33, 272–277 (1980). https://doi.org/10.1007/BF02414752

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02414752

Keywords