Determination and localization of oil components in living benthic organisms by fluorescence microscopy
- E. Zeeck1
© Biologische Anstalt Helgoland 1980
Microspectrofluorometric measurements are made to determine uptake and distribution of oil in marine organisms after exposure to crude oil. Equipment combining fluorescence microscopy with spectral analysis of the fluorescence emission is described. After contamination with oil, the intestine content ofLumbricillus lineatus, Nereis diversicolor andAnaitides mucosa shows a fluorescence emission at long wavelengths with a maximum at about 550 nm; this is in contrast to the fluorescence emission of these organisms without oil contamination. There is evidence that aromatic hydrocarbons are metabolized in the intestine of the worms studied.