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A lectin histochemical study on carbohydrate moieties of the gonadotropin-like substance in the epithelial cells of Hatschek's pit ofBranchiostoma belcheri
Helgoländer Meeresuntersuchungen volume 51, pages 53–59 (1997)
Abstract
The present light microscopic lectin, histochemical study suggests for the first time that the vertebrate gonadotropin-like substance in the basal part of the epithelial cells of Hatschek's pit is a sialic acid-containing glycoprotein. The binding intensity of the epithelial cells in Hatschek's pit to 6 lectins (Limulus polyphemus agglutinin (LPA), Wheat germ agglutinin (WGA),Helix pomatia agglutinin (HPA), Concanavalin A (Con A),Ulex europaeus agglutinin I (UEA I) andRicinus communis agglutinin I (RCA I)) indicate that the carbohydrate composition of the gonadotrophic glycoprotein is similar to that of mammals and fish, and that N-acetyl-D-galactosamine, sialic acid, glucosamine, D-mannose and L-fucose are components of the carbohydrate portion.
Literature Cited
Araujo, A. C. G., Souto-Padron, T. & Souza, W. de, 1993. Cytochemical localization of carbohydrate residues in microfilariae ofWuchereria bancrofti andBrugia malayi.—Cytochemistry41, 571–578
Burzawa-Gerard, E. & Fontaine, Y. A., 1972. The gonadotropins of lower vertebrates.—Gen. comp. Endocrinol. (Suppl)3, 715–728.
Chang, C. Y., Zhu, Y. T. & Cheng, D. Y., 1982. Immunocytochemical demonstration of luteinizing hormone (LH) in Hatschek's pit of amphioxus (Branchiostoma belcheri Gray).—Kexue Tongbao27, 1233–1234.
Debray, H., Decout, D., Strecher, G., Spik, G. & Montreuil, J., 1981. Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins.—Eur. J. Biochem.117, 41.
Fang, Y. Q., 1993. Immunocytochemical localization of fish gonadotropin (GTH) in the brain vesicle and Hatschek's pit of amphioxus.—Chinese Sci. Bull.38, 1747–1751.
Fang, Y. Q. & Qi, X., 1989. Ultrastructural study of the Hatschek's pit epithelial cells of amphioxus. —Sci. China (B)32, 1465–1472.
Fang, Y. Q. & Wang, L., 1984. The preliminary study of homogenate of the wheel organ and Hatschek's pit of amphioxus on testicular development in young toad (Bufo malanostictus).—Acta Biol. exp. sin.17, 115–117.
Goldstein, I. J. & Poretz, R. D., 1986. Isolation, physicochemical characterization, and carbohydratebinding specificity of lectins. In: The lectins. Ed. by I. E. Liener, N. Sharon & I. J. Goldstein. Acad. Press Orlando, Fla, 33.
Grimek, H. J., Nuti, L. C., Nuti, K. M. & McShan, W. H., 1976. Effect of neuraminidase treatment on the biological activity of highly purified ovine FSH and LH in hypophysectomized immature male and female rats.—Endocrinology98, 105–110.
Grimek, H. J., Gorski, J. & Wentworth, B. C., 1979. Purification and characterization of bovine follicle-stimulating hormone: Comparison with ovine follicle-stimulating hormone.—Endocrinology104, 140–147.
Hammarström, S., Murghy, L. A., Goldstein, I. J. & Etzler, M. E., 1977, Carbohydrate binding specificity of four N-acetyl-D-galactosamine-“specific” lectins:Helix pomatia A hemagglutinin, Soybean agglutinin, Lima bean lectin, andDolichos biflorus lectin.—Biochemistry16, 2750.
Komuro, M., 1987. Presence of glycoconjugates in prolactin granules of male rats.—Cell Tissue Res.250, 181–184.
Komuro, M. & Shioda, T., 1981. Localization of sialic acid-containing hormones in GTH cell and ACTH cell of the rat anterior pituitary.—Cell Tissue Res.220, 519–528.
Mazzuca, M., Roche, A. C., Lhermitte, M. & Koussel, P., 1977.Limulus polyphemus lectin site in human bronchial mucosa.—J. Histochem. Cytochem.25, 470–473.
Nielsen, C., 1995. Animal evolution. Oxford Univ. Press., London, 467 pp.
Nozaki, M. & Gorbman, A., 1992. The question of functional homology of Hatschek's pit of amphioxus (Branchiostoma belcheri) and the vertebrate adenohypophysis.—Zool. Sci.9, 387–395.
Perez, J. & Fernandez-Lebrez, P., 1989. Immuno- and lectin-electron-microscopic investigation of the neural lobe of the hypophysis in the snakeNatrix maura.—Cell Tissue Res.258, 547–554.
Quatacker J. R., Annaert, W. G. & De Potter, W. P., 1990. Detection of sialic acid residues in the axonal reticulum of rat superior cervical ganglion cells by lectin-gold cytochemistry.—J. Histochem. Cytochem.38, 1445–1449.
Roche, A. C., Schauer, R. & Monsigny, M., 1975. Protein-sugar interactions purification by affinity chromatography of limulin and N-acylneur-aminidyl-binding protein.—Febs Lett.57, 245–249.
Spicer, S. S. & Schulte, B. A., 1992. Diversity of cell glycoconjugates shown histochemically: A perspective. —J. Histochem. Cytochem.40, 1–38.
Sugii, S., Kabat, E. A. & Baer, H. H., 1982. further immunochemical studies on the combining sites ofLotus tetragonolobus andUlex europaeus I and II lectins.—Carbohydr. Res.99, 99.
Tjoa, L. T. & Welsch, U., 1974. Electron microscopical observation on Kölliker's and hatschek's pit and on the wheel organ in the head region of amphioxus (Branchiostoma lanceolatum).—Cell Tissue Res.153, 175–187.
Yamada, K. & Shimizu, S., 1979. The use of peroxidase-labelledLimulus polyphemus agglutinin for the histochemistry of sialic acid-containing glycoproteins in light microscopy.—Histochem. J.11, 457–471.
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Fang, Y.Q., Welsch, U. A lectin histochemical study on carbohydrate moieties of the gonadotropin-like substance in the epithelial cells of Hatschek's pit ofBranchiostoma belcheri . Helgoländer Meeresunters. 51, 53–59 (1997). https://doi.org/10.1007/BF02908753
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DOI: https://doi.org/10.1007/BF02908753