Fig. 2
From: Formation and discharge of nematocysts is controlled by a proton gradient across the cyst membrane
Approaches to stain acidic compartments. a, b Living acontia of Aiptasia sp. treated in sea water with LysoSensor (TM) Blue DND-167 (1:100 dilution) for 30 min. a Interference contrast, b same preparation, UV-fluorescence. c, d Living ephyrae of Aurelia aurita treated in seawater with LysoSensor (1:100 dilution) for 6 days. c Interference contrast, d same preparation, UV-fluorescence. Note that nematocysts generally appear unstained (black) in a fluorescent environment. e, f Crystals of poly-DL-aspartic acid in diluted hydrochloric acid, stained with LysoSensor (1:100 dilution) for 30 min. e Interference contrast, f same preparation, UV-fluorescence. Note that the crystals appear unstained (black) in a fluorescent environment. g, h CM-Sephadex equilibrated with 0.5 M sodium phosphate buffer (g) at pH 3.0 and (h) at pH 4.0, treated with LysoSensor