Fig. 3

Staining with phalloidin, propodium idodide and serotonin-immunoreactivity. Sagittal (a) and frontal view (b) of the rhinophore double labelled with serotonin-immunoreactivity (green) and phalloidin (red). Serotonergic fibres (SF) project from the rhinophore ganglion (RG) to the rhinophore lobes (RL). The epithelial cell layer of the rhinophore lobe (RL) is stained with phalloidin indicating that the cell processes on the surface are cilia (C). Phalloidin-labelled muscle bundles (M) extend only in longitudinal direction. c-f: Double labelling with the serotonin-immunoreactivity (green) and propidium iodide (red). The RG and side branches are surrounded by many cell nuclei (c, e). Single SF project into the RL (e, f). The small lymphatic channels (LC) are associated with serotonin-immunoreactivity (f, arrow). Scale bars: a-d = 500 μm, e, f = 100 μm