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Table 2 Marenzelleria spp. Restriction sites of the restriction enzymes used for species identification

From: Molecular identification key based on PCR/RFLP for three polychaete sibling species of the genus Marenzelleria, and the species’ current distribution in the Baltic Sea

 

COI

bp 342–347

ApaI

Marenzelleria viXridis

5′ GGGCC↓C…3′

Marenzelleria neglecta

5′ GGACCT…3′

Marenzelleria arctia

5′ GGACCT…3′

 

16S

bp 225–230

SspI

Marenzelleria neglecta

5′ AAT↓ATT…3′

Marenzelleria viridis

5′ AATATA…3′

Marenzelleria arctia

5′ AGCATA…3′

 

16S

bp 236–241

AseI

Marenzelleria arctia

5′ AT↓TAAT…3′

Marenzelleria neglecta

5′ ACTAAT…3′

Marenzelleria viridis

5′ ACTAAT…3′

  

5′ ACTAAC…3′

  1. The indicated position of the restriction sites refers to the positions in the aligned sequences