The effect of re-dissolution solvents and HPLC columns on the analysis of mycosporine-like amino acids in the eulittoral macroalgae Prasiola crispa and Porphyra umbilicalis

Table 1 MAA separation and concentrations in extracts of the green alga Prasiola crispa after re-dissolution in different solvents and after using different HPLC columns

	Concentration (mg g⁻¹ DW)
Column	Solvent	MAA-1 (324 nm)	MAA-2 (324 nm)	MAA-3 (324 nm)	MAA-4 (324 nm)	Total
Synergi C18	Methanol	1.22 ± 0.1 (7.7 min)	0.64 ± 0.07 (17.2 min)	0.14 ± 0.01 (17.5 min)	0.12 ± 0.02	2.12 ± 0.19
Synergi C18	Water	ND	4.91 ± 0.12 (7.6 min)	ND	0.77 ± 0.02 (17.5 min)	5.68 ± 0.14
Synergi C18	HPLC eluent	ND	4.87 ± 0.12 (7.6 min)	ND	1.05 ± 0.25 (17.5 min)	5.92 ± 0.21
Sphereclone C8	Methanol	1.84 ± 0.16 (3.4 min)	0.06 ± 0.01 (4.4 min)	0.14 ± 0.01 (4.9 min)	ND	2.04 ± 0.18
Sphereclone C8	Water	4.70 ± 0.14 (3.5 min)	0.90 ± 0.04 (4.6 min)	ND	ND	5.6 ± 0.21
Sphereclone C8	HPLC eluent	4.85 ± 0.32 (3.5 min)	1.27 ± 0.26 (4.6 min)	ND	ND	6.12 ± 0.17
Luna C8	Methanol	^a				<0.5
Luna C8	Water	^a				<0.5
Luna C8	HPLC eluent	^a				<0.5

The absorbance maxima of the different MAAs and the retention time in minutes of the respective peaks is given in brackets. Data given represent mean value ± SD (n = 3). ND not detected
^aMixed unidentifiable peaks

ISSN: 1438-3888