Table 2 MAA separation and concentrations in extracts of the red alga Porphyra umbilicalis after re-dissolution in different solvents and after using different HPLC columns
Column | Concentration (mg g−1 DW) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Solvent | Palythine | MAA-5 | Shinorine | MAA-6 | MAA-7 | Porphyra-334 | MAA-8 | MAA-9 | Total | |
(320 nm) | (324 nm) | (333 nm) | (334 nm) | (334 nm) | (334 nm) | (334 nm) | (322 nm) | |||
Synergi C18 | Methanol | a | ND | 0.30 ± 0.01 (5.5 min) | 1.36 ± 0.27 (6.5 min) | ND | 4.26 ± 0.08 (7.8 min) | ND | ND | 6.05 ± 0.34 |
Synergi C18 | Water | 0.21 ± 0.01 (2.9 min) | 0.22 ± 0.02 (4.1 min) | 0.63 ± 0.04 (5.4 min) | ND | ND | 6.90 ± 0.04 (7.7 min) | ND | ND | 8.01 ± 0.05 |
Synergi C18 | HPLC eluent | 0.21 ± 0.01 (2.9 min) | 0.22 ± 0.02 (4.1 min) | 0.64 ± 0.03 (5.4 min) | ND | ND | 6.83 ± 0.05 (7.7 min) | ND | ND | 7.88 ± 0.06 |
Sphereclone C8 | Methanol | 0.18 ± 0.02 (5.0 min) | ND | 0.15 ± 0.01 (3.1 min) | ND | 3.49 ± 0.11 (3.6 min) | 1.59 ± 0.10 (3.8 min) | 0.78 ± 0.04 (4.0 min) | 0.11 ± 0.02 (6.2 min) | 6.54 ± 0.26 |
Sphereclone C8 | Water | 0.22 ± 0.01 (5.0 min) | ND | 0.62 ± 0.03 (3.1 min) | ND | ND | 6.95 ± 0.11 (3.8 min) | ND | 0.11 ± 0.01 (6.4 min) | 8.00 ± 0.06 |
Sphereclone C8 | HPLC eluent | 0.23 ± 0.04 (5.0 min) | ND | 0.61 ± 0.02 (3.1 min) | ND | ND | 6.87 ± 0.07 (3.8 min) | ND | 0.12 ± 0.01 (6.4 min) | 7.90 ± 0.10 |
Luna C8 | Methanol | a | ND | 0.40 ± 0.02 (6.0 min) | ND | ND | 5.40 ± 0.20 (8.8 min) | ND | ND | 5.97 ± 0.24 |
Luna C8 | Water | 0.29 ± 0.02 (2.5 min) | 0.16 ± 0.01 (3.7 min) | 0.62 ± 0.05 (6.0 min) | ND | ND | 8.52 ± 0.66 (8.9 min) | ND | ND | 9.59 ± 0.73 |
Luna C8 | HPLC eluent | 0.21 ± 0.01 (2.5 min) | 0.12 ± 0.01 (3.7 min) | 0.31 ± 0.01 (6.0 min) | ND | ND | 6.08 ± 0.16 (8.9 min) | ND | ND | 6.72 ± 0.19 |