Table 3 The analytical HPLC set-ups available from the literature for the separation of MAAs with an emphasis on the re-dissolution solvents used

100% methanol

Isocratic/C8

0.1% acetic acid; 25% aqueous methanol

Dunlap and Chalker (1986), Banaszak et al. (2006)

100% methanol

Isocratic/C8

0.1% acetic acid; 25% aqueous methanol

Neale et al. (1988), Karsten et al. (1998)

100% methanol

Isocratic/C8

0.1% acetic acid; 80% aqueous methanol

Moisan and Mitchell (2001)

100% methanol

Isocratic/C8

0.1% acetic acid; 5% aqueous methanol

Figueroa et al. (2003)

100% methanol

Isocratic/C8

0.1% acetic acid; 2.5% aqueous methanol

Korbee et al. (2005), Karsten et al. (2005), de la Coba et al. (2008)

80% methanol

Isocratic/C8

0.1% acetic acid; 55% aqueous methanol

Carroll and Shick (1996)

25% methanol

Isocratic/C8

0.1% acetic acid; 25% aqueous methanol

Tartarotti and Sommaruga (2002)

Deionized water

Isocratic/C8

0.1% acetic acid; 25% aqueous methanol

Shick et al. (1999)

80:20 methanol:THF

Gradient/C18

From 0.2% acetic acid to 0.1% acetic acid; 25% aqueous methanol

Dionisio-Sese et al. (1997)

100% water

Gradient/C18

From 0.2% acetic acid to 0.1% acetic acid; 25% methanol; 25% acetonitrile

Caretto et al. (2001)

0.2% aqueous trifluoroacetic acid/ammonium acetate

Gradient/two coupled C18 columns

From 0.2% trifluoroacetic acid/ammonium hydroxide in water to 0.2% trifluoro-acetic acid/ammonium hydroxide in water:methanol; acetonitrile (80:10:10)

Caretto et al. (2005)