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Table 1 Oligonucleotide probes used in this study

From: Culturable heterotrophic bacteria from the marine sponge Dendrilla nigra: isolation and phylogenetic diversity of actinobacteria

Probes

Target (positions)

Sequences 5′ → 3′

EUB338a

Bacterial 16S rRNA (338–335)

GCT GCC TCC CGT AGG AGT

NON338b

Negative control

ACT CCT ACG GGA GGC AGC

ALT734c

MSI026 16S rRNA (734–754)

GTGTGGGTAGCGAACAGGAT

MAR82c

MSI032 16S rRNA (82–102)

AATACCCATGAGGCTTGACG

MIC703c

MSI033 16S rRNA (703–723)

TCCACGCTCTCAAAGGAACT

SAC605c

MSI039 16S rRNA (605–625)

TCGATACTGACGCTGAGGTG

ROS46c

MSI042 16S rRNA (46–66)

GTAAAGCTCCTTCGCCTGTG

ACT792c

MSI051 16S rRNA (792–812)

ACTAGGTGTGGGCGACATTC

PSE171c

MSI057 16S rRNA (171–191)

ACCAACAGAATAAGCACCGG

  1. aBacterial 16S rRNA (338–355) specific for all eubacteria
  2. bNegative control probe
  3. cProbe name indicates the phylogenetic group closet to each isolate based on 16S rRNA sequences of the respective isolate, and numbers indicate the position of the first base in the 16S rRNA gene (Escherichia coli numbering system) for which the probe was designed to target
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Helgoland Marine Research

ISSN: 1438-3888

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