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Table 1 Medium DSMZ #621 and derivatives; metal composition M44 originates from Medium #590

From: Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

Medium DSMZ

Quantity

Notes

#621 + M44

#621

#621 − Glu

Peptone (Bacto)

0.25 g

pH 7,5 autoclaved

+

+

+

Yeast extract (Bacto)

0.25 g

+

+

+

Aged seawater

724.00 ml

+

725.00 ml

+

Distilled water

241.00 ml

+

+

251.00 ml

Mineral salt solution

19.00 ml

Sterile filtered

+

+

+

Vitamin sol. (double conc.)

5.00 ml

Sterile filtered

+

+

+

Metals 44 (DSMZ #590)

1.00 ml

Sterile filtered

+

+

Glucose sol. (2, 5%)

10.00 ml

Sterile filtered

+

+

  1. Both media are provided by the German collection of microorganisms and cell cultures (www.dsmz.de). Ingredients marked with negative symbol were replaced by distilled water