Fig. 3
From: The male reproductive system of Hippolyte inermis Leach 1815 (Decapoda, Caridea)
Vas deferens. a Diagram of vas deferens. b Frontal section of the proximal vas deferens (first coil) PAS stain. Scale bar, 200 μm. c Sagital section of the proximal vas deferens (second coil) Resorcine-Fuchsine stain. Scale bar, 50 μm. d SEM microphotograph of paraffin-carved sections of the medial vas deferens. Scale bar, 120 μm. e Sagital semithin section of the medial vas deferens. Toluidine stain. Scale bar, 100 μm. f Frontal section (only one coil) of distal vas deferens. Resorcine—Fuchsine stain. Scale bar, 20 μm. g Detail from E. Scale bar, 10 μm. h Frontal semithin section of the distal vas deferens. Toluidine stain. Scale bar, 50 μm. i Transverse semithin section of the distal vas deferens, near to gonopore. Toluidine stain. Scale bar, 100 μm. j Sagital section of the distal vas deferens near to gonopore. Mallory’s trichrome stain. Scale bar, 100 μm. k Detail from J. Scale bar, 20 μm. ag androgenic gland, arrowheads spermatozoa, ct cuticle, dvd distal vas deferens, e epithelium, f folder, fb fibrilar layer, g gut, go gonopore, 1th h first coil, 2th h second coil, m muscle, ml muscular layer; mvd median vas deferens, pvd proximal vas deferens, s 1 protein substance, s 2 mucous substance, t teste