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Table 1 Criteria used to define maturity stages in male and female R. clavata, adapted from Zeiner and Wolf (1993)

From: Reproductive biology of Raja clavata (Elasmobranchii: Rajidae) from Southern Black Sea coast around Turkey

Stage

Male characteristics

Female characteristics

Immature

No coiling of vas deferens

Testes small and undeveloped

Claspers do not extend past posterior edge of pelvic fin

Small undeveloped ovaries and nidamental glands

No differentiated ova present

Maturing

Some coiling of vas deferens

Testes enlarging

Claspers extend past pelvic fin edge but are only somewhat calcified

Ovaries beginning to enlarge and differentiate

Ova white, distinguishable but small

Nidamental glands widening but still gelatinous in appearance

Mature

Complete coiling of vas deferens

Large developed testes

Calcified claspers

Large yellow (yolked) ova, greater than 2.0 cm in diameter

Large, solid, functional nidamental glands