Fig. 2

Karyotype of G. loreto (a, c, e, g) and G. brasiliensis (b, d, f, h). Conventional Giemsa staining (a, b), highlighting Ag-NORs sites on the short arms of pairs 3 and 4 of G. loreto and G. brasiliensis, respectively; C-banding showing heterochromatin in centromeric chromosomal regions (c, d); Sequential staining with fluorochromes CMA₃/DAPI (e, f), showing the composition of most GC-rich heterochromatin blocks and location of ribosomal genes by dual-color FISH with rDNA 18S (pair 3 of G. loreto and pair 4 of G. brasiliensis) and 5S (pairs 9 and 14 of G. loreto and pairs 10 and 15 of G. brasiliensis) (g, h) probes. Bar = 5 μm