Seasonal variations in larval biomass and biochemical composition of brown shrimp, Crangon crangon (Decapoda, Caridea), at hatching

Table 1 Crangon crangon, newly hatched larvae

Biomass parameters	Factor	SS	df	MS	F
W (μg)	Month	2303.63	7	329.09	297.69***
	Hatch (month)	209.89	24	8.75	7.91*
	Error	106.13	96	1.11
	Total	2619.64	127
C (μg)	Month	451.692	7	64.527	281.23***
	Hatch (month)	42.972	24	1.791	7.80*
	Error	22.027	96	0.229
	Total	516.691	127
N (μg)	Month	38.3862	7	5.4837	299.67***
	Hatch (month)	2.8055	24	0.1169	6.39*
	Error	1.7568	96	0.0183
	Total	42.9484	127
H (μg)	Month	14.0591	7	2.0084	363.88***
	Hatch (month)	1.0340	24	0.0431	7.81*
	Error	0.5299	96	0.0055
	Total	15.6230	127
Lipid (μg)	Month	8.1994	7	1.1713	323.39***
	Hatch (month)	0.6374	24	0.0266	7.33*
	Error	0.5795	160	0.0036
	Total	9.4163	191
Protein (μg)	Month	182.220	7	26.031	1011.6***
	Hatch (month)	2.697	24	0.112	4.4*
	Error	4.117	160	0.026
	Total	189.034	191
E (J)	Month	0.63117	7	0.090167	388.65***
	Hatch (month)	0.06995	24	0.002914	12.56*
	Error	0.03722	160	0.000232
	Total	0.73834	191
C (% W)	Month	56.5	7	8.1	10.8*
	Hatch (Month)	109.5	24	4.6	6.1*
	Error	72.1	96	0.8
	Total	238.1	127
N (% W)	Month	34.69	7	4.96	36.88*
	Hatch (month)	6.87	24	0.29	2.13*
	Error	12.90	96	0.13
	Total	54.46	127
H (% W)	Month	53.864	7	7.695	163.7**
	Hatch (month)	10.731	24	0.447	9.5*
	Error	4.512	96	0.047
	Total	69.107	127
C/N ratio	Month	0.970	7	0.139	14.8*
	Hatch (month)	0.809	24	0.034	3.6*
	Error	0.896	96	0.009
	Total	2.675	127
Lipid (% W)	Month	4.987	7	0.712	23.9*
	Hatch (month)	5.124	24	0.214	7.2*
	Error	4.765	160	0.030
	Total	14.877	191
Protein (% W)	Month	32.8	7	4.7	37*
	Hatch (month)	8.4	24	0.3	3*
	Error	20.3	160	0.1
	Total	61.4	191
E (J*mg W⁻¹)	Month	30.00	7	4.29	15.8*
	Hatch (month)	31.23	24	1.30	4.81*
	Error	43.02	160	0.27
	Total	104.25	191

Two-level nested ANOVA evaluating differences in dry weight, elemental composition and proximate biochemical composition among months and hatches nested within months; interaction between these factors; biomass parameters: (W) dry weight, (C) contents of carbon, (N) nitrogen, (H) hydrogen, C/N ratio, lipid, protein and (E) energy; significant differences marked with asterisks (* P < 0.05; ** P < 0.01; *** P < 0.001)

ISSN: 1438-3888