|
Biomass parameters
|
Factor
|
SS
|
df
|
MS
|
F
|
|---|
|
W (μg)
|
Month
|
2303.63
|
7
|
329.09
|
297.69***
|
| |
Hatch (month)
|
209.89
|
24
|
8.75
|
7.91*
|
| |
Error
|
106.13
|
96
|
1.11
| |
| |
Total
|
2619.64
|
127
| | |
|
C (μg)
|
Month
|
451.692
|
7
|
64.527
|
281.23***
|
| |
Hatch (month)
|
42.972
|
24
|
1.791
|
7.80*
|
| |
Error
|
22.027
|
96
|
0.229
| |
| |
Total
|
516.691
|
127
| | |
|
N (μg)
|
Month
|
38.3862
|
7
|
5.4837
|
299.67***
|
| |
Hatch (month)
|
2.8055
|
24
|
0.1169
|
6.39*
|
| |
Error
|
1.7568
|
96
|
0.0183
| |
| |
Total
|
42.9484
|
127
| | |
|
H (μg)
|
Month
|
14.0591
|
7
|
2.0084
|
363.88***
|
| |
Hatch (month)
|
1.0340
|
24
|
0.0431
|
7.81*
|
| |
Error
|
0.5299
|
96
|
0.0055
| |
| |
Total
|
15.6230
|
127
| | |
|
Lipid (μg)
|
Month
|
8.1994
|
7
|
1.1713
|
323.39***
|
| |
Hatch (month)
|
0.6374
|
24
|
0.0266
|
7.33*
|
| |
Error
|
0.5795
|
160
|
0.0036
| |
| |
Total
|
9.4163
|
191
| | |
|
Protein (μg)
|
Month
|
182.220
|
7
|
26.031
|
1011.6***
|
| |
Hatch (month)
|
2.697
|
24
|
0.112
|
4.4*
|
| |
Error
|
4.117
|
160
|
0.026
| |
| |
Total
|
189.034
|
191
| | |
|
E (J)
|
Month
|
0.63117
|
7
|
0.090167
|
388.65***
|
| |
Hatch (month)
|
0.06995
|
24
|
0.002914
|
12.56*
|
| |
Error
|
0.03722
|
160
|
0.000232
| |
| |
Total
|
0.73834
|
191
| | |
|
C (% W)
|
Month
|
56.5
|
7
|
8.1
|
10.8*
|
| |
Hatch (Month)
|
109.5
|
24
|
4.6
|
6.1*
|
| |
Error
|
72.1
|
96
|
0.8
| |
| |
Total
|
238.1
|
127
| | |
|
N (% W)
|
Month
|
34.69
|
7
|
4.96
|
36.88*
|
| |
Hatch (month)
|
6.87
|
24
|
0.29
|
2.13*
|
| |
Error
|
12.90
|
96
|
0.13
| |
| |
Total
|
54.46
|
127
| | |
|
H (% W)
|
Month
|
53.864
|
7
|
7.695
|
163.7**
|
| |
Hatch (month)
|
10.731
|
24
|
0.447
|
9.5*
|
| |
Error
|
4.512
|
96
|
0.047
| |
| |
Total
|
69.107
|
127
| | |
|
C/N ratio
|
Month
|
0.970
|
7
|
0.139
|
14.8*
|
| |
Hatch (month)
|
0.809
|
24
|
0.034
|
3.6*
|
| |
Error
|
0.896
|
96
|
0.009
| |
| |
Total
|
2.675
|
127
| | |
|
Lipid (% W)
|
Month
|
4.987
|
7
|
0.712
|
23.9*
|
| |
Hatch (month)
|
5.124
|
24
|
0.214
|
7.2*
|
| |
Error
|
4.765
|
160
|
0.030
| |
| |
Total
|
14.877
|
191
| | |
|
Protein (% W)
|
Month
|
32.8
|
7
|
4.7
|
37*
|
| |
Hatch (month)
|
8.4
|
24
|
0.3
|
3*
|
| |
Error
|
20.3
|
160
|
0.1
| |
|
|
Total
|
61.4
|
191
| | |
|
E (J*mg W−1)
|
Month
|
30.00
|
7
|
4.29
|
15.8*
|
| |
Hatch (month)
|
31.23
|
24
|
1.30
|
4.81*
|
| |
Error
|
43.02
|
160
|
0.27
| |
| |
Total
|
104.25
|
191
| | |
- Two-level nested ANOVA evaluating differences in dry weight, elemental composition and proximate biochemical composition among months and hatches nested within months; interaction between these factors; biomass parameters: (W) dry weight, (C) contents of carbon, (N) nitrogen, (H) hydrogen, C/N ratio, lipid, protein and (E) energy; significant differences marked with asterisks (* P < 0.05; ** P < 0.01; *** P < 0.001)
