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Table 2 Chemical properties of the experimental seawater used for the fertilization experiments

From: CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic

Experiment Treatment Salinity (‰) A T (μmol kg−1 SW) pH* (NIST) C T (μmol kg−1 SW) pHC (Total/NIST scale) pCO2 (µatm)
Fertilization
 Test 1
~180 31.3 ± 0.1 2,419.5 ± 0.7 8.09 ± 0.00 2,165.0 ± 5.9 8.34 ± 0.01 193 ± 7.5
~380 31.3 ± 0.1 2,396.1 ± 2.8 7.98 ± 0.00 (§) 8.21 ± 0.00 351 ± 0.6
~550–800 31.2 ± 0.1 2,404.5 ± 0.7 7.82 ± 0.01 2,346.5 ± 3.6 7.85 ± 0.01 683 ± 22.9
~1,300 31.3 ± 0.1 2,408.5 ± 0.7 7.78 ± 0.03 2,406.2 ± 8.6 7.66 ± 0.04 1,089 ± 101.0
Fertilization
 Test 2
~180 30.5 ± 0.1 2,423.3 ± 24.0 8.09 ± 0.00 2,152.0 ± 11.1 8.38 ± 0.03 175 ± 11.3
~380 30.7 ± 0.2 2,363.0 ± 2.8 7.9 ± 0.01 2,247.3 ± 8.1 8.03 ± 0.02 425 ± 23.9
~550–800 30.6 ± 0.4 2,398.8 ± 3.5 7.8 ± 0.01 2,322.0 ± 3.0 7.92 ± 0.00 582 ± 1.9
~1,300 30.6 ± 0.2 2,393.3 ± 1.4 (−) 2,419.0 ± 2.8 7.57 ± 0.01 1,354 ± 22.4
~2,000 30.3 ± 0.0 2,429.3 ± 2.8 7.58 ± 0.01 2,504.6 ± 8.1 7.42 ± 0.02 1,968 ± 99.9
Fertilization
 Test 3
~180 30.3 ± 0.1 2,380.0 ± 5.7 8.1 ± 0.01 2,149.3 ± 15.9 8.31 ± 0.03 207 ± 17.9
~380 30.1 ± 0.1 2,361.5 ± 2.1 7.93 ± 0.01 2,247.0 ± 11.1 8.04 ± 0.04 423 ± 39.9
~550–800 30.1 ± 0.1 2,362.0 ± 1.4 7.76 ± 0.02 2,321.8 ± 2.7 7.80 ± 0.01 758 ± 11.9
~1,300 30.6 ± 0.3 2,364.0 ± 2.8 7.74 ± 0.01 2,416.3 ± 4.9 7.48 ± 0.01 1,644 ± 37.9
~2,000 30.6 ± 0.3 2,411.1 ± 16.3 7.59 ± 0.12 2,506.5 ± 3.6 7.36 ± 0.05 2,244 ± 244.0
Fertilization
 Test 4
~180 30.8 ± 0.1 2,415.6 ± 2.8 7.9 ± 0.01 2,184.5 ± 26.4 8.30 ± 0.07 218 ± 40.2
~380 30.8 ± 0.1 2,406.1 ± 3.5 7.87 ± 0.01 2,257.5 ± 6.1 8.11 ± 0.01 352 ± 9.5
~550–800 30.6 ± 0.1 2,396.0 ± 0.7 7.69 ± 0.00 (§) 7.90 ± 0.00 769 ± 0.3
~1,300 30.9 ± 0.1 2,446.0 ± 2.1 7.68 ± 0.01 2,444.2 ± 23.4 7.66 ± 0.10 1,118 ± 272.0
  1. For calculations: Input conditions 20 °C, 1 d bars pressure, P and Si: 0 µmol kg−1 SW. Identical output conditions except for temperature (experimental temperature: 3 °C)
  2. Values represent mean ± SD, n = 2–3 ASW subsamples per treatment. Measured conditions represent pre-industrial (~180 µatm), present (~380 µatm) and acidified scenarios (~550–800, ~1,300 µatm or higher)
  3. pH*: A T integrated pH measurement. pHC: pH calculated, in total scale except C T values were not available or were inconsistent to the treatment used (§), in which case calculations were made using A T and pH* with NIST scale (values in bold)