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Table 2 Chemical properties of the experimental seawater used for the fertilization experiments

From: CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic

Experiment

Treatment

Salinity (‰)

A T (μmol kg−1 SW)

pH* (NIST)

C T (μmol kg−1 SW)

pHC (Total/NIST scale)

pCO2 (µatm)

Fertilization

 Test 1

~180

31.3 ± 0.1

2,419.5 ± 0.7

8.09 ± 0.00

2,165.0 ± 5.9

8.34 ± 0.01

193 ± 7.5

~380

31.3 ± 0.1

2,396.1 ± 2.8

7.98 ± 0.00

(§)

8.21 ± 0.00

351 ± 0.6

~550–800

31.2 ± 0.1

2,404.5 ± 0.7

7.82 ± 0.01

2,346.5 ± 3.6

7.85 ± 0.01

683 ± 22.9

~1,300

31.3 ± 0.1

2,408.5 ± 0.7

7.78 ± 0.03

2,406.2 ± 8.6

7.66 ± 0.04

1,089 ± 101.0

Fertilization

 Test 2

~180

30.5 ± 0.1

2,423.3 ± 24.0

8.09 ± 0.00

2,152.0 ± 11.1

8.38 ± 0.03

175 ± 11.3

~380

30.7 ± 0.2

2,363.0 ± 2.8

7.9 ± 0.01

2,247.3 ± 8.1

8.03 ± 0.02

425 ± 23.9

~550–800

30.6 ± 0.4

2,398.8 ± 3.5

7.8 ± 0.01

2,322.0 ± 3.0

7.92 ± 0.00

582 ± 1.9

~1,300

30.6 ± 0.2

2,393.3 ± 1.4

(−)

2,419.0 ± 2.8

7.57 ± 0.01

1,354 ± 22.4

~2,000

30.3 ± 0.0

2,429.3 ± 2.8

7.58 ± 0.01

2,504.6 ± 8.1

7.42 ± 0.02

1,968 ± 99.9

Fertilization

 Test 3

~180

30.3 ± 0.1

2,380.0 ± 5.7

8.1 ± 0.01

2,149.3 ± 15.9

8.31 ± 0.03

207 ± 17.9

~380

30.1 ± 0.1

2,361.5 ± 2.1

7.93 ± 0.01

2,247.0 ± 11.1

8.04 ± 0.04

423 ± 39.9

~550–800

30.1 ± 0.1

2,362.0 ± 1.4

7.76 ± 0.02

2,321.8 ± 2.7

7.80 ± 0.01

758 ± 11.9

~1,300

30.6 ± 0.3

2,364.0 ± 2.8

7.74 ± 0.01

2,416.3 ± 4.9

7.48 ± 0.01

1,644 ± 37.9

~2,000

30.6 ± 0.3

2,411.1 ± 16.3

7.59 ± 0.12

2,506.5 ± 3.6

7.36 ± 0.05

2,244 ± 244.0

Fertilization

 Test 4

~180

30.8 ± 0.1

2,415.6 ± 2.8

7.9 ± 0.01

2,184.5 ± 26.4

8.30 ± 0.07

218 ± 40.2

~380

30.8 ± 0.1

2,406.1 ± 3.5

7.87 ± 0.01

2,257.5 ± 6.1

8.11 ± 0.01

352 ± 9.5

~550–800

30.6 ± 0.1

2,396.0 ± 0.7

7.69 ± 0.00

(§)

7.90 ± 0.00

769 ± 0.3

~1,300

30.9 ± 0.1

2,446.0 ± 2.1

7.68 ± 0.01

2,444.2 ± 23.4

7.66 ± 0.10

1,118 ± 272.0

  1. For calculations: Input conditions 20 °C, 1 d bars pressure, P and Si: 0 µmol kg−1 SW. Identical output conditions except for temperature (experimental temperature: 3 °C)
  2. Values represent mean ± SD, n = 2–3 ASW subsamples per treatment. Measured conditions represent pre-industrial (~180 µatm), present (~380 µatm) and acidified scenarios (~550–800, ~1,300 µatm or higher)
  3. pH*: A T integrated pH measurement. pHC: pH calculated, in total scale except C T values were not available or were inconsistent to the treatment used (§), in which case calculations were made using A T and pH* with NIST scale (values in bold)
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Helgoland Marine Research

ISSN: 1438-3888

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