Environmental variables, habitat discontinuity and life history shaping the genetic structure of Pomatoschistus marmoratus

Table 1 Characterization of the eight microsatellite loci used to genotype five populations of Pomatoschistus marmoratus from the Mar Menor coastal lagoon and adjacent marine sites (SE Spain)

Locus	Reference	Repeat sequence	Primer sequences (5′–3′) and the fluorescent dye to mark the 5′ end of the forward primer	Annealing temperature (°C)	Alleles	Size range (bp)
Pmar03	Berrebi et al. (2006)	(AC)₈GC(AC)₁₂	F: FAM-AGGTTTCCGCTGTTACTGCGAC R: GAGATAACAAGCGCAAAGTCC	57	20	314–350
Pmar05	Berrebi et al. (2006)	(GT)₆GC(GT)₁₆	F: FAM-TCTCCGTGGCTCTCCCCAGTGC R: CTGATCAGGGCCACAGCATCT	54	18	218–248
Pmar08	Berrebi et al. (2006)	(ATCTA)₅	F: NED-GTCTGGTCAATACTGAACAGCTC R: ACAGTCTCCAACGGCCGTTCAG	61	8	232–252
Pmin04	Larmuseau et al. (2007)	(GT)₇ACA(TG)₈	F: FAM-TCTAACAGGCAGCTGAACGA R: GGCTCAAACACACACGAAAA	57	15	209–231
Pmin09	Larmuseau et al. (2007)	(TG)₂CG(TG)₂₀	F: HEX-GCCGTGGATGCATTATCAGT R: GGGATGTGTGAGTGTGCAAG	57	16	196–222
Pmin29	Larmuseau et al. (2007)	(CA)₉	F: NED-GGGCTCCACTTTGTTAGCAG R: CGTGGGAATTCCTTGATTGT	55	5	206–210
Pmin35	Larmuseau et al. (2007)	(CA)₃TA(CA)₂₀	F: HEX-GTGACTGGGAGCGTTTGAGT R: GCCCTATCTGCCTGACAAAG	55	2	162–168
Pmin38	Larmuseau et al. (2007)	(TG)₄TA(TG)₅	F: FAM-TGAATCCGAAGCCTGGTAAC R: TCCCTTCTGCTTCCTTTTGA	54	31	194–258

Note: The loci Pmar03, Pmin04 and Pmin09 were amplified by multiplex PCR with an annealing temperature of 57 °C. The loci Pmin29 and Pmin35 were amplified by multiplex PCR with an annealing temperature of 55 °C. The loci Pmar05 and Pmin38, despite amplified using the same annealing temperature (54°), were not used as multiplex PCR

ISSN: 1438-3888