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Fig. 3 | Helgoland Marine Research

Fig. 3

From: Morphology and ultrastructure of the adult ovarian cycle in Mithracidae (Crustacea, Decapoda, Brachyura, Majoidea)

Fig. 3

Ovarian histology at the DE stage; ac, gi stained with hematoxylin and eosin; d xylidine ponceau stain; e, j mercuric-bromophenol blue stain; f, k PAS stain; l Alcian blue stain; a Mithrax tortugae (Rathbun, 1920), oocyte with large central nucleus, surrounded by follicular cells showing mature yolk vesicles arranged in the oocyte cortex. b Mithrax hispidus (Herbst, 1790). Note the cytoplasmic vesicles in the perinuclear region (white arrow) and lucid vesicles between the yolk vesicles (black arrow); c Omalacantha bicornuta (Latreille, 1825), oocyte cortex without chorion (black arrow); df Mithrax hispidus, Mithrax tortugae and Mithraculus forceps A. Milne-Edwards, 1875, cytoplasm and yolk vesicles with positive reactivity for proteins and neutral polysaccharides; g Mithraculus forceps, more developed oocytes with perinuclear space filled by mature yolk vesicles; h Mithrax hispidus, oocyte cortex with thin chorion (black arrow); i Omalacantha bicornuta, rounded central nucleus; j, k Mithrax tortugae and Mithraculus forceps, cytoplasm and yolk vesicles with positive reactivity for proteins and neutral polysaccharides; l Mithrax hispidus, cytoplasm and yolk vesicles with the absence of acid polysaccharides. C chorion, FC follicular cell, GZ germinal zone, N nucleus, Nu nucleolus, Y yolk vesicle

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