Fig. 5
Light microscopy of the MAT stage; a–e hematoxylin and eosin stain; f mercuric-bromophenol blue stain; g xylidine ponceau stain; h PAS stain; i Alcian blue stain; a–c Mithrax tortugae (Rathbun, 1920), Mithrax hispidus (Herbst, 1790) and Mithraculus forceps A. Milne-Edwards, 1875, oocyte showing irregular nucleus and cytoplasm filled with mature yolk vesicles. Note the elliptical and flattened follicular cells; d, e Mithrax tortugae and Mithraculus forceps, acidophilic chorion juxtaposed follicular cells; f, g Mithrax tortugae and Omalacantha bicornuta (Latreille, 1825), chorion and yolk vesicles reactive for proteins; h Mithrax tortugae, chorion and yolk vesicles reactive for neutral polysaccharides; i Omalacantha bicornuta chorion and yolk vesicles with the absence of acid polysaccharides. Note the glycogen granules (white arrow) among the yolk vesicles. C chorion, FC follicular cell, N nucleus, Y yolk vesicles