Skip to main content

Table 2 Statistical evaluation of response variables of M. stellatus and C. crispus: amplitudes of the maximum quantum yield (Fv/Fm) for the high-light stress phase and the recovery phase as well as Chl a concentration, ratios of pigments (β-carotene/Chl a, lutein/Chl a, phycoerythrin/Chl a, phycocyanin/Chl a) and antioxidant (antioxidants (TE)/Chl a), total lipid content, saturation states of fatty acids [sum of saturated fatty acids (SFA), sum of monounsaturated fatty acids (MUFA), sum of polyunsaturated fatty acids (PUFA), saturated/unsaturated fatty acid ratio (SFA/UFA)] and shorter-chain/longer-chain fatty acid ratio (C14 + C16/C18 + C20) of initial thallus branches, using two-factorial analysis of variance, with the factors sampling event (October 2011, March 2012, May 2012 and August 2012) and isolate (Mast-ex, Mast-ov and Chon-ov) and their interaction

From: Fatty acid compositions associated with high-light tolerance in the intertidal rhodophytes Mastocarpus stellatus and Chondrus crispus

Source of variation Fv/Fm amplitude (high-light stress phase) Fv/Fm amplitude (recovery phase)
SS df MS F-ratio p value SS df MS F-ratio p value
Isolate 0.052 2 0.026 8.193 0.001 0.029 2 0.015 3.003 0.059
Sampling event 0.276 3 0.092 29.233 <0.001 0.140 3 0.047 9.589 <0.001
Isolate × sampling event 0.091 6 0.015 4.799 0.001 0.063 6 0.011 2.167 0.063
Error 0.151 48 0.003    0.234 48 0.005   
Source of variation Chl a β-carotene/Chl a
SS df MS F-ratio p value SS df MS F-ratio p value
Isolate 0.115 2 0.057 32.101 <0.001 3.0 × 10−5 2 1.5 × 10−5 0.545 0.583
Sampling event 0.016 3 0.005 2.985 0.040 2.2 × 10−4 3 7.3 × 10−5 2.646 0.060
Isolate × sampling event 0.019 6 0.003 1.786 0.122 0.001 6 1.3 × 10−4 4.586 0.001
Error 0.086 48 0.002    0.001 48 2.8 × 10−5   
Source of variation Lutein/Chl a Phycoerythrin/Chl a
SS df MS F-ratio p value SS df MS F-ratio p value
Isolate 0.021 2 0.011 44.316 <0.001 0.273 2 0.137 0.115 0.891
Sampling event 0.011 3 0.004 14.937 <0.001 1.988 3 0.663 0.560 0.644
Isolate × sampling event 0.004 6 0.001 2.731 0.023 6.260 6 1.043 0.882 0.516
Error 0.012 48 2.4 × 10−4    56.805 48 1.183   
Source of variation Phycocyanin/Chl a Antioxidants (TE)/Chl a
SS df MS F-ratio p value SS df MS F-ratio p value
Isolate 0.188 2 0.094 2.790 0.071 106.940 2 53.470 49.380 <0.001
Sampling event 0.142 3 0.047 1.402 0.254 137.450 3 45.817 42.312 <0.001
Isolate × sampling event 0.081 6 0.014 0.401 0.875 30.891 6 5.149 4.755 0.001
Error 1.618 48 0.034    51.976 48 1.083   
Source of variation Total lipid SFA
SS df MS F-ratio p value SS df MS F-ratio p value
Isolate 0.001 2 3.4 × 10−4 44.667 <0.001 0.006 2 0.003 1.428 0.250
Sampling event 1.8 × 10−5 3 6.1 × 10−6 0.815 0.492 0.023 3 0.008 3.468 0.023
Isolate × sampling event 3.2 × 10−4 6 5.3 × 10−5 7.037 <0.001 0.028 6 0.005 2.071 0.074
Error 3.6 × 10−4 48 7.5 × 10−6    0.108 48 0.002   
Source of variation MUFA PUFA
SS df MS F-ratio p value SS df MS F-ratio p value
Isolate 0.011 2 0.006 16.058 <0.001 0.001 2 3.4 × 10−4 0.055 0.947
Sampling event 0.005 3 0.002 5.250 0.003 0.079 3 0.026 4.332 0.009
Isolate × sampling event 0.005 6 0.001 2.277 0.052 0.036 6 0.006 0.987 0.445
Error 0.017 48 3.8 × 10−4    0.293 48 0.006   
Source of variation SFA/UFA C14 + C16/C18 + C20
SS df MS F-ratio p value SS df MS F-ratio p value
Isolate 0.002 2 0.001 0.801 0.455 3.4 × 10−5 2 1.7 × 10−4 4.073 0.023
Sampling event 0.177 3 0.059 55.952 <0.001 0.001 3 3.9 × 10−4 93.871 <0.001
Isolate × sampling event 0.018 6 0.003 2.857 0.019 3.9 × 10−5 6 6.4 × 10−6 1.550 0.183
Error 0.050 47 0.001    2.0 × 10−4 47 4.1 × 10−6   
  1. p values in bold highlight significant differences at p < 0.05
  2. Mast-ex, Mastocarpus stellatus from higher levels of the lower intertidal; Mast-ov, Mastocarpus stellatus from overlapping zone in deeper levels of lower intertidal; Chon-ov, Chondrus crispus from overlapping zone in deeper levels of lower intertidal