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Table 2 Statistical evaluation of response variables of M. stellatus and C. crispus: amplitudes of the maximum quantum yield (Fv/Fm) for the high-light stress phase and the recovery phase as well as Chl a concentration, ratios of pigments (β-carotene/Chl a, lutein/Chl a, phycoerythrin/Chl a, phycocyanin/Chl a) and antioxidant (antioxidants (TE)/Chl a), total lipid content, saturation states of fatty acids [sum of saturated fatty acids (SFA), sum of monounsaturated fatty acids (MUFA), sum of polyunsaturated fatty acids (PUFA), saturated/unsaturated fatty acid ratio (SFA/UFA)] and shorter-chain/longer-chain fatty acid ratio (C14 + C16/C18 + C20) of initial thallus branches, using two-factorial analysis of variance, with the factors sampling event (October 2011, March 2012, May 2012 and August 2012) and isolate (Mast-ex, Mast-ov and Chon-ov) and their interaction

From: Fatty acid compositions associated with high-light tolerance in the intertidal rhodophytes Mastocarpus stellatus and Chondrus crispus

Source of variation

Fv/Fm amplitude (high-light stress phase)

Fv/Fm amplitude (recovery phase)

SS

df

MS

F-ratio

p value

SS

df

MS

F-ratio

p value

Isolate

0.052

2

0.026

8.193

0.001

0.029

2

0.015

3.003

0.059

Sampling event

0.276

3

0.092

29.233

<0.001

0.140

3

0.047

9.589

<0.001

Isolate × sampling event

0.091

6

0.015

4.799

0.001

0.063

6

0.011

2.167

0.063

Error

0.151

48

0.003

  

0.234

48

0.005

  

Source of variation

Chl a

β-carotene/Chl a

SS

df

MS

F-ratio

p value

SS

df

MS

F-ratio

p value

Isolate

0.115

2

0.057

32.101

<0.001

3.0 × 10−5

2

1.5 × 10−5

0.545

0.583

Sampling event

0.016

3

0.005

2.985

0.040

2.2 × 10−4

3

7.3 × 10−5

2.646

0.060

Isolate × sampling event

0.019

6

0.003

1.786

0.122

0.001

6

1.3 × 10−4

4.586

0.001

Error

0.086

48

0.002

  

0.001

48

2.8 × 10−5

  

Source of variation

Lutein/Chl a

Phycoerythrin/Chl a

SS

df

MS

F-ratio

p value

SS

df

MS

F-ratio

p value

Isolate

0.021

2

0.011

44.316

<0.001

0.273

2

0.137

0.115

0.891

Sampling event

0.011

3

0.004

14.937

<0.001

1.988

3

0.663

0.560

0.644

Isolate × sampling event

0.004

6

0.001

2.731

0.023

6.260

6

1.043

0.882

0.516

Error

0.012

48

2.4 × 10−4

  

56.805

48

1.183

  

Source of variation

Phycocyanin/Chl a

Antioxidants (TE)/Chl a

SS

df

MS

F-ratio

p value

SS

df

MS

F-ratio

p value

Isolate

0.188

2

0.094

2.790

0.071

106.940

2

53.470

49.380

<0.001

Sampling event

0.142

3

0.047

1.402

0.254

137.450

3

45.817

42.312

<0.001

Isolate × sampling event

0.081

6

0.014

0.401

0.875

30.891

6

5.149

4.755

0.001

Error

1.618

48

0.034

  

51.976

48

1.083

  

Source of variation

Total lipid

SFA

SS

df

MS

F-ratio

p value

SS

df

MS

F-ratio

p value

Isolate

0.001

2

3.4 × 10−4

44.667

<0.001

0.006

2

0.003

1.428

0.250

Sampling event

1.8 × 10−5

3

6.1 × 10−6

0.815

0.492

0.023

3

0.008

3.468

0.023

Isolate × sampling event

3.2 × 10−4

6

5.3 × 10−5

7.037

<0.001

0.028

6

0.005

2.071

0.074

Error

3.6 × 10−4

48

7.5 × 10−6

  

0.108

48

0.002

  

Source of variation

MUFA

PUFA

SS

df

MS

F-ratio

p value

SS

df

MS

F-ratio

p value

Isolate

0.011

2

0.006

16.058

<0.001

0.001

2

3.4 × 10−4

0.055

0.947

Sampling event

0.005

3

0.002

5.250

0.003

0.079

3

0.026

4.332

0.009

Isolate × sampling event

0.005

6

0.001

2.277

0.052

0.036

6

0.006

0.987

0.445

Error

0.017

48

3.8 × 10−4

  

0.293

48

0.006

  

Source of variation

SFA/UFA

C14 + C16/C18 + C20

SS

df

MS

F-ratio

p value

SS

df

MS

F-ratio

p value

Isolate

0.002

2

0.001

0.801

0.455

3.4 × 10−5

2

1.7 × 10−4

4.073

0.023

Sampling event

0.177

3

0.059

55.952

<0.001

0.001

3

3.9 × 10−4

93.871

<0.001

Isolate × sampling event

0.018

6

0.003

2.857

0.019

3.9 × 10−5

6

6.4 × 10−6

1.550

0.183

Error

0.050

47

0.001

  

2.0 × 10−4

47

4.1 × 10−6

  
  1. p values in bold highlight significant differences at p < 0.05
  2. Mast-ex, Mastocarpus stellatus from higher levels of the lower intertidal; Mast-ov, Mastocarpus stellatus from overlapping zone in deeper levels of lower intertidal; Chon-ov, Chondrus crispus from overlapping zone in deeper levels of lower intertidal