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Table 3 Pigment and lipid composition of M. stellatus and C. crispus: chlorophyll a concentration, ratios of pigments to chlorophyll a (β-carotene/Chl a, lutein/Chl a, phycoerythrin/Chl a, phycocyanin/Chl a), total lipid content, saturation states of fatty acids [sum of saturated fatty acids (SFA), sum of monounsaturated fatty acids (MUFA), sum of polyunsaturated fatty acids (PUFA), saturated/unsaturated fatty acid ratio (SFA/UFA)] and shorter-chain/longer-chain fatty acid ratio (C14 + C16/C18 + C20) of initial thallus branches collected on four sampling events (October 2011, March 2012, May 2012 and August 2012)

From: Fatty acid compositions associated with high-light tolerance in the intertidal rhodophytes Mastocarpus stellatus and Chondrus crispus

Response variables of initial thallus branches

Sampling event

October 2011

March 2012

May 2012

August 2012

Chl a (µg mg−1 dry weight)

 Mast-ex

0.31 ± 0.01

0.26 ± 0.01

0.30 ± 0.01

0.31 ± 0.02

 Mast-ov

0.23 ± 0.02

0.26 ± 0.01

0.28 ± 0.01

0.22 ± 0.01

 Chon-ov

0.32 ± 0.03

0.33 ± 0.01

0.40 ± 0.04

0.35 ± 0.03

β-carotene/Chl a

 Mast-ex

0.11 ± 0.00a

0.11 ± 0.00a

0.12 ± 0.00a

0.11 ± 0.00b

 Mast-ov

0.11 ± 0.00a

0.11 ± 0.00a

0.12 ± 0.00a

0.11 ± 0.00b

 Chon-ov

0.11 ± 0.00a

0.11 ± 0.00a

0.11 ± 0.00b

0.12 ± 0.00a

Lutein/Chl a

 Mast-ex

0.21 ± 0.01a

0.23 ± 0.00a

0.25 ± 0.01a

0.23 ± 0.00a

 Mast-ov

0.19 ± 0.01b

0.20 ± 0.01b

0.24 ± 0.01a

0.23 ± 0.00a

 Chon-ov

0.17 ± 0.01b

0.19 ± 0.01b

0.18 ± 0.01b

0.20 ± 0.01b

Phycoerythrin/Chl a

 Mast-ex

1.48 ± 0.29

1.72 ± 0.12

2.05 ± 0.23

2.11 ± 0.72

 Mast-ov

1.70 ± 0.30

2.82 ± 0.95

1.48 ± 0.28

2.01 ± 0.78

 Chon-ov

2.24 ± 0.33

1.76 ± 0.36

1.47 ± 0.19

2.10 ± 0.47

Phycocyanin/Chl a

 Mast-ex

0.27 ± 0.05

0.26 ± 0.02

0.30 ± 0.04

0.37 ± 0.10

 Mast-ov

0.30 ± 0.04

0.41 ± 0.12

0.24 ± 0.04

0.39 ± 0.15

 Chon-ov

0.45 ± 0.08

0.47 ± 0.09

0.32 ± 0.03

0.50 ± 0.11

Total lipid (% of dry weight)

 Mast-ex

1.37 ± 0.15b

1.21 ± 0.14b

1.19 ± 0.05b

1.62 ± 0.24a

 Mast-ov

1.11 ± 0.08b

1.01 ± 0.07b

1.21 ± 0.03b

1.12 ± 0.04b

 Chon-ov

1.69 ± 0.10a

1.87 ± 0.06a

1.70 ± 0.04a

1.31 ± 0.12b

SFA (mass% of total fatty acids)

 Mast-ex

35.6 ± 0.7

30.4 ± 0.2

32.0 ± 0.2

37.6 ± 0.7

 Mast-ov

34.4 ± 0.3

30.6 ± 0.2

32.1 ± 0.1

37.1 ± 0.8

 Chon-ov

36.3 ± 1.4

30.4 ± 0.4

32.0 ± 0.3

34.4 ± 0.4

MUFA (mass% of total fatty acids)

 Mast-ex

10.4 ± 1.5

13.2 ± 0.2

11.3 ± 0.1

9.5 ± 1.3

 Mast-ov

13.7 ± 0.7

13.5 ± 0.1

10.6 ± 0.1

10.4 ± 0.2

 Chon-ov

9.7 ± 0.4

8.6 ± 0.3

9.9 ± 0.2

9.4 ± 0.2

PUFA (mass% of total fatty acids)

 Mast-ex

54.0 ± 0.9

56.4 ± 0.1

56.7 ± 0.2

53.0 ± 0.7

 Mast-ov

52.0 ± 0.4

55.9 ± 0.2

57.3 ± 0.2

52.5 ± 1.0

 Chon-ov

54.1 ± 1.2

61.0 ± 0.4

58.1 ± 0.3

56.2 ± 0.2

SFA/UFA

 Mast-ex

0.55 ± 0.02ab

0.44 ± 0.00a

0.47 ± 0.00a

0.60 ± 0.02a

 Mast-ov

0.52 ± 0.01b

0.44 ± 0.00a

0.47 ± 0.00a

0.59 ± 0.02a

 Chon-ov

0.57 ± 0.03a

0.44 ± 0.01a

0.47 ± 0.01a

0.52 ± 0.01b

C14 + C16/C18 + C20

 Mast-ex

0.58 ± 0.02

0.46 ± 0.00

0.49 ± 0.00

0.62 ± 0.02

 Mast-ov

0.56 ± 0.01

0.47 ± 0.00

0.49 ± 0.00

0.60 ± 0.02

 Chon-ov

0.52 ± 0.01

0.42 ± 0.01

0.46 ± 0.01

0.50 ± 0.01

  1. Table shows mean ± SEM (n = 5). Dark circles indicate isolate-specific (Mast-ex, Mast-ov and Chon-ov) differences within all four sampling events that are significant at p < 0.05 (significant isolate effect of 2-way ANOVA followed by a Fisher’s LSD test). Different letters (a and b) indicate significant differences among algal isolates within one of the four sampling events (significant interaction isolate × sampling event of 2-way ANOVA followed by a Fisher’s LSD test, p < 0.05)
  2. Mast-ex, Mastocarpus stellatus from higher levels of the lower intertidal; Mast-ov, Mastocarpus stellatus from overlapping zone in deeper levels of lower intertidal; Chon-ov, Chondrus crispus from overlapping zone in deeper levels of lower intertidal; SEM, standard error of the mean