|
Response variables of initial thallus branches
|
Sampling event
|
|---|
|
October 2011
|
March 2012
|
May 2012
|
August 2012
|
|---|
|
Chl a (µg mg−1 dry weight)●
|
|
Mast-ex
|
0.31 ± 0.01
|
0.26 ± 0.01
|
0.30 ± 0.01
|
0.31 ± 0.02
|
|
Mast-ov
|
0.23 ± 0.02
|
0.26 ± 0.01
|
0.28 ± 0.01
|
0.22 ± 0.01
|
|
Chon-ov
|
0.32 ± 0.03
|
0.33 ± 0.01
|
0.40 ± 0.04
|
0.35 ± 0.03
|
|
β-carotene/Chl a
|
|
Mast-ex
|
0.11 ± 0.00a
|
0.11 ± 0.00a
|
0.12 ± 0.00a
|
0.11 ± 0.00b
|
|
Mast-ov
|
0.11 ± 0.00a
|
0.11 ± 0.00a
|
0.12 ± 0.00a
|
0.11 ± 0.00b
|
|
Chon-ov
|
0.11 ± 0.00a
|
0.11 ± 0.00a
|
0.11 ± 0.00b
|
0.12 ± 0.00a
|
|
Lutein/Chl a
|
|
Mast-ex
|
0.21 ± 0.01a
|
0.23 ± 0.00a
|
0.25 ± 0.01a
|
0.23 ± 0.00a
|
|
Mast-ov
|
0.19 ± 0.01b
|
0.20 ± 0.01b
|
0.24 ± 0.01a
|
0.23 ± 0.00a
|
|
Chon-ov
|
0.17 ± 0.01b
|
0.19 ± 0.01b
|
0.18 ± 0.01b
|
0.20 ± 0.01b
|
|
Phycoerythrin/Chl a
|
|
Mast-ex
|
1.48 ± 0.29
|
1.72 ± 0.12
|
2.05 ± 0.23
|
2.11 ± 0.72
|
|
Mast-ov
|
1.70 ± 0.30
|
2.82 ± 0.95
|
1.48 ± 0.28
|
2.01 ± 0.78
|
|
Chon-ov
|
2.24 ± 0.33
|
1.76 ± 0.36
|
1.47 ± 0.19
|
2.10 ± 0.47
|
|
Phycocyanin/Chl a
|
|
Mast-ex
|
0.27 ± 0.05
|
0.26 ± 0.02
|
0.30 ± 0.04
|
0.37 ± 0.10
|
|
Mast-ov
|
0.30 ± 0.04
|
0.41 ± 0.12
|
0.24 ± 0.04
|
0.39 ± 0.15
|
|
Chon-ov
|
0.45 ± 0.08
|
0.47 ± 0.09
|
0.32 ± 0.03
|
0.50 ± 0.11
|
|
Total lipid (% of dry weight)
|
|
Mast-ex
|
1.37 ± 0.15b
|
1.21 ± 0.14b
|
1.19 ± 0.05b
|
1.62 ± 0.24a
|
|
Mast-ov
|
1.11 ± 0.08b
|
1.01 ± 0.07b
|
1.21 ± 0.03b
|
1.12 ± 0.04b
|
|
Chon-ov
|
1.69 ± 0.10a
|
1.87 ± 0.06a
|
1.70 ± 0.04a
|
1.31 ± 0.12b
|
|
SFA (mass% of total fatty acids)
|
|
Mast-ex
|
35.6 ± 0.7
|
30.4 ± 0.2
|
32.0 ± 0.2
|
37.6 ± 0.7
|
|
Mast-ov
|
34.4 ± 0.3
|
30.6 ± 0.2
|
32.1 ± 0.1
|
37.1 ± 0.8
|
|
Chon-ov
|
36.3 ± 1.4
|
30.4 ± 0.4
|
32.0 ± 0.3
|
34.4 ± 0.4
|
|
MUFA (mass% of total fatty acids)●
|
|
Mast-ex
|
10.4 ± 1.5
|
13.2 ± 0.2
|
11.3 ± 0.1
|
9.5 ± 1.3
|
|
Mast-ov
|
13.7 ± 0.7
|
13.5 ± 0.1
|
10.6 ± 0.1
|
10.4 ± 0.2
|
|
Chon-ov
|
9.7 ± 0.4
|
8.6 ± 0.3
|
9.9 ± 0.2
|
9.4 ± 0.2
|
|
PUFA (mass% of total fatty acids)
|
|
Mast-ex
|
54.0 ± 0.9
|
56.4 ± 0.1
|
56.7 ± 0.2
|
53.0 ± 0.7
|
|
Mast-ov
|
52.0 ± 0.4
|
55.9 ± 0.2
|
57.3 ± 0.2
|
52.5 ± 1.0
|
|
Chon-ov
|
54.1 ± 1.2
|
61.0 ± 0.4
|
58.1 ± 0.3
|
56.2 ± 0.2
|
|
SFA/UFA
|
|
Mast-ex
|
0.55 ± 0.02ab
|
0.44 ± 0.00a
|
0.47 ± 0.00a
|
0.60 ± 0.02a
|
|
Mast-ov
|
0.52 ± 0.01b
|
0.44 ± 0.00a
|
0.47 ± 0.00a
|
0.59 ± 0.02a
|
|
Chon-ov
|
0.57 ± 0.03a
|
0.44 ± 0.01a
|
0.47 ± 0.01a
|
0.52 ± 0.01b
|
|
C14 + C16/C18 + C20●
|
|
Mast-ex
|
0.58 ± 0.02
|
0.46 ± 0.00
|
0.49 ± 0.00
|
0.62 ± 0.02
|
|
Mast-ov
|
0.56 ± 0.01
|
0.47 ± 0.00
|
0.49 ± 0.00
|
0.60 ± 0.02
|
|
Chon-ov
|
0.52 ± 0.01
|
0.42 ± 0.01
|
0.46 ± 0.01
|
0.50 ± 0.01
|
- Table shows mean ± SEM (n = 5). Dark circles indicate isolate-specific (Mast-ex, Mast-ov and Chon-ov) differences within all four sampling events that are significant at p < 0.05 (significant isolate effect of 2-way ANOVA followed by a Fisher’s LSD test). Different letters (a and b) indicate significant differences among algal isolates within one of the four sampling events (significant interaction isolate × sampling event of 2-way ANOVA followed by a Fisher’s LSD test, p < 0.05)
- Mast-ex, Mastocarpus stellatus from higher levels of the lower intertidal; Mast-ov, Mastocarpus stellatus from overlapping zone in deeper levels of lower intertidal; Chon-ov, Chondrus crispus from overlapping zone in deeper levels of lower intertidal; SEM, standard error of the mean
