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Table 4 Fatty acid compositions of M. stellatus and C. crispus: single fatty acids (mass% of total fatty acids) of initial thallus branches collected on four sampling events (October 2011, March 2012, May 2012 and August 2012)

From: Fatty acid compositions associated with high-light tolerance in the intertidal rhodophytes Mastocarpus stellatus and Chondrus crispus

Fatty acid

Sampling event

October 2011

March 2012

May 2012

August 2012

14:0

 Mast-ex

2.8 ± 0.1

1.5 ± 0.0

1.8 ± 0.0

3.4 ± 0.1

 Mast-ov

2.9 ± 0.1

1.8 ± 0.0

1.9 ± 0.0

3.2 ± 0.1

 Chon-ov

3.2 ± 0.2

1.8 ± 0.1

2.4 ± 0.1

3.4 ± 0.1

16:0

 Mast-ex

30.5 ± 0.5a

26.8 ± 0.1a

28.2 ± 0.1a

32.0 ± 0.7a

 Mast-ov

29.0 ± 0.3b

26.7 ± 0.1a

28.2 ± 0.1a

31.2 ± 0.6a

 Chon-ov

30.4 ± 0.6a

27.2 ± 0.3a

28.2 ± 0.2a

29.5 ± 0.6b

16:1(n-7)

 Mast-ex

2.4 ± 0.0a

2.3 ± 0.0a

1.8 ± 0.0a

2.0 ± 0.1a

 Mast-ov

2.6 ± 0.1a

2.3 ± 0.0a

1.7 ± 0.0a

1.8 ± 0.1a

 Chon-ov

0.4 ± 0.2b

0.6 ± 0.0b

0.7 ± 0.1b

0.4 ± 0.0b

18:0

 Mast-ex

1.2 ± 0.1

1.0 ± 0.1

0.9 ± 0.0

1.3 ± 0.2

 Mast-ov

1.1 ± 0.0

1.0 ± 0.1

1.1 ± 0.1

1.4 ± 0.2

 Chon-ov

2.2 ± 0.6

1.3 ± 0.2

1.1 ± 0.1

1.3 ± 0.1

18:1(n-7)

 Mast-ex

2.2 ± 0.0

2.4 ± 0.1

2.4 ± 0.1

2.0 ± 0.0

 Mast-ov

1.9 ± 0.1

2.2 ± 0.1

2.1 ± 0.0

1.4 ± 0.1

 Chon-ov

1.2 ± 0.2

1.6 ± 0.1

1.5 ± 0.1

0.9 ± 0.0

18:1(n-9)

 Mast-ex

5.6 ± 1.4b

8.0 ± 0.1ab

6.7 ± 0.0a

5.3 ± 1.3b

 Mast-ov

8.6 ± 0.6a

8.5 ± 0.1a

6.5 ± 0.0a

6.9 ± 0.2ab

 Chon-ov

8.0 ± 0.1a

6.3 ± 0.2b

7.6 ± 0.2a

8.0 ± 0.2a

18:2(n-6)

    

 Mast-ex

1.1 ± 0.1

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

 Mast-ov

1.5 ± 0.2

1.1 ± 0.0

0.9 ± 0.0

1.0 ± 0.0

 Chon-ov

1.4 ± 0.2

2.3 ± 0.3

1.6 ± 0.1

1.6 ± 0.6

20:4(n-6)

 Mast-ex

25.3 ± 0.9b

16.5 ± 0.5b

13.8 ± 0.4c

25.0 ± 0.4ab

 Mast-ov

24.4 ± 0.5b

13.4 ± 0.7c

15.9 ± 0.3b

23.7 ± 0.4b

 Chon-ov

27.4 ± 0.4a

25.8 ± 0.9a

24.8 ± 1.0a

25.9 ± 0.4a

20:5(n-3)

 Mast-ex

25.9 ± 0.5a

37.0 ± 0.6b

40.0 ± 0.4a

25.8 ± 0.4b

 Mast-ov

24.1 ± 0.4a

39.4 ± 0.9a

38.7 ± 0.4a

26.0 ± 0.7b

 Chon-ov

24.5 ± 1.5a

32.8 ± 1.0c

31.1 ± 0.7b

28.1 ± 0.1a

  1. Table shows mean ± SEM (n = 5, with exception of n = 4 for Chon-ov collected in August 2012). The nomenclature of fatty acids (a:b(n-x)) is defined as follows: a = number of C-atoms (chain length), b = number of double bonds and (n-x) = position of the first double bond relative to the methyl-end. Dark circles indicate isolate-specific (Mast-ex, Mast-ov and Chon-ov) differences within all four sampling events that are significant at p < 0.05 (significant isolate effect of 2-way ANOVA followed by a Fisher’s LSD test). Different letters (a, b and c) indicate significant differences among algal isolates within one of the four sampling events (significant interaction isolate × sampling event of 2-way ANOVA followed by a Fisher’s LSD test, p < 0.05)
  2. Mast-ex, Mastocarpus stellatus from higher levels of the lower intertidal; Mast-ov, Mastocarpus stellatus from overlapping zone in deeper levels of lower intertidal; Chon-ov, Chondrus crispus from overlapping zone in deeper levels of lower intertidal; SEM, standard error of the mean