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Table 5 Statistical evaluation of fatty acid compositions of M. stellatus and C. crispus: single fatty acids of initial thallus branches, using two-factorial analysis of variance, with the factors sampling event (October 2011, March 2012, May 2012 and August 2012) and isolate (Mast-ex, Mast-ov and Chon-ov) and their interaction

From: Fatty acid compositions associated with high-light tolerance in the intertidal rhodophytes Mastocarpus stellatus and Chondrus crispus

Source of variation FA 14:0 FA 16:0 FA 16:1(n-7)
SS df MS F p SS df MS F p SS df MS F p
Isolate 2.7 × 10−5 2 1.3 × 10−5 1.992 0.148 0.001 2 3.3 × 10−4 3.255 0.047 0.003 2 0.001 175.034 <0.001
Sampling event 0.002 3 0.001 84.675 <0.001 0.016 3 0.005 51.590 <0.001 1.1 × 10−4 3 3.6 × 10−5 4.188 0.010
Isolate × sampling event 1.6 × 10−5 6 2.7 × 10−6 0.409 0.869 0.002 6 3.8 × 10−4 3.710 0.004 2.5 × 10−4 6 4.1 × 10−5 4.808 0.001
Error 3.2 × 10−4 47 6.7 × 10−6    0.005 47 1.0 × 10−4    4.0 × 10−4 47 8.5 × 10−6   
Source of variation FA 18:0 FA 18:1(n-7) FA 18:1(n-9)
SS df MS F p SS df MS F p SS df MS F p
Isolate 2.3 × 10−4 2 1.1 × 10−4 4.199 0.021 4.8 × 10−4 2 2.4 × 10−4 21.566 <0.001 0.002 2 0.001 4.239 0.020
Sampling event 5.9 × 10−5 3 2.0 × 10−5 0.720 0.545 3.1 × 10−4 3 1.0 × 10−4 9.365 <0.001 0.001 3 1.8 × 10−4 0.929 0.434
Isolate × sampling event 2.9 × 10−4 6 4.8 × 10−5 1.787 0.122 1.3 × 10−4 6 2.2 × 10−5 2.007 0.084 0.004 6 0.001 3.850 0.003
Error 0.001 47 2.7 × 10−5    0.001 47 1.1 × 10−5    0.009 47 1.9 × 10−4   
Source of variation FA 18:2(n-6) FA 20:4(n-6) FA 20:5(n-3)
SS df MS F p SS df MS F p SS df MS F p
Isolate 0.001 2 2.9 × 10−4 12.315 <0.001 0.053 2 0.027 125.717 <0.001 0.013 2 0.007 22.987 <0.001
Sampling event 3.5 × 10−5 3 1.2 × 10−5 0.493 0.689 0.075 3 0.025 118.942 <0.001 0.191 3 0.064 221.290 <0.001
Isolate × sampling event 2.7 × 10−4 6 4.5 × 10−5 1.916 0.098 0.028 6 0.005 22.157 <0.001 0.029 6 0.005 16.761 <0.001
Error 0.001 47 2.4 × 10−5    0.010 47 2.1 × 10−4    0.014 47 2.9 × 10−4   
  1. p values in bold highlight significant differences at p < 0.05. The nomenclature of fatty acids (a:b(n-x)) is defined as follows: a = number of C-atoms (chain length), b = number of double bonds and (n-x) = position of the first double bond relative to the methyl-end
  2. Mast-ex, Mastocarpus stellatus from higher levels of the lower intertidal; Mast-ov, Mastocarpus stellatus from overlapping zone in deeper levels of lower intertidal; Chon-ov, Chondrus crispus from overlapping zone in deeper levels of lower intertidal