Fig. 4From: Culture of benthic diatom Nitzschia sp. with macroalgae carriers and its application as feed of juveniles Stichopus japonicusEffects of carrier treatment methods on diatom culture. Method A: Ulva powder and M-f/2 culture medium were sterilized separately at 121 °C for 30 min.. Method B: Ulva powder and M-f/2 culture medium were mixed and then sterilized at 121 °C for 30 min. Method C: Ulva powder was added to sterilized M-f/2 culture medium after drying at 80 °CBack to article page