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Fig. 1 | Helgoland Marine Research

Fig. 1

From: Methods to study organogenesis in decapod crustacean larvae II: analysing cells and tissues

Fig. 1

Macro- and microphotography, and autofluorescence-based imaging of decapod larvae (see section "Reflected-light-based methods: macro- and micro-photography" and "Autofluorescence-based imaging"). a Macrophoto of a Carcinus maenas Zoea IV using the camera setup shown in panel e) (M. Hörnig, unpublished). b1, b2 Autofluorescence image of a Carcinus maenas Zoea IV mounted on a microscopic slide in ethanol and cover-slipped using modelling clay as spacers (F. Spitzner, unpublished). The image was taken using a Nikon Eclipse 90i upright microscope (excitation 359–371 nm; emission 379 nm; see panel d equipped with a digital camera. Image b2 was black-white inverted using image processing software. Note that more details of the setae on the exopods of the maxillipeds are visible. c Two-channel image of Zoea 1 of Xantho poressa combining brightfield image with chlorophyll autofluorescence in the intestine after feeding with diatoms (red) (modified from [33]). d Fully motorised upright fluorescent microscope Nikon Eclipse 90i (image S. Harzsch). e Macrophotographic setup (image M. Hörnig)

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