Fig. 12

Visualisations of malacostracan developmental stages based on µCT (all images J. Krieger, unpublished; see section "X-ray microscopy (µCT)" for details). a Based on a µCT-dataset of a Zoea II of Carcinus maenas, the outer surface (here, virtually sliced along the midline in grey) can be visualised (“Isosurface“-module in Amira®; compare Table 2) in addition to a volume rendering (“Volren-module“ in Amira®) showing internal elements of the musculature, stomach as well as the compound eye (orange colourmap). b Two-dimensional virtual slice through that larva along the dorso-ventral body-axis as indicated by the dashed line in a). Note that the space between cuticle and internal elements is an artefact resulting from tissue shrinking due to chemical fixation. Differences in the tissue densities, however, allow the discrimination for manual segmentation of desired organs based on the resulting different greyscales. c µCT-scanned female of the amphipod Caprella mutica (“Volume-rendering“ in grey) and 21 post-larvae (Manca-stage) located inside the marsupium (“Volume-rendering“ in orange based on the manual segmentation of the interior marsupial cavity (in higher magnification in c1). d, d1, d2: one post-larva (orange) was randomly selected and manually segmentated and also its nervous system reconstructed (green in d and d1). Using manual segmentation, a single juvenile can be either highlighted among its 20 siblings (d), or visualised as isolated individual for detailed anatomical analysis (d1 and d2)