Fig. 15
From: Methods to study organogenesis in decapod crustacean larvae II: analysing cells and tissues
Immunohistochemical analysis of dissected whole mounts of the central nervous system from larvae of Birgus latro (see section "Immunohistochemistry" for details). a Dissected central nervous system of a Zoea 4 labelled with an antiserum against synaptic proteins to visualise the neuropil areas (T. Kirchhoff, unpublished), black–white inverted confocal laser-scan image. b Double labelling of a ventral nerve cord of a Zoea 2 to show areas of synaptic neuropil (magenta) and FMRFamide-like neuropeptides (green; T. Kirchhoff, unpublished). c Confocal laser-scan microscope Leica SP5 (image S. Harzsch). MD: neuromere of mandible, MX 1, 2: neuromeres of maxilla 1 and 2, T1, 8: thoracic neuromeres 1, 8, P1, P5: pleomeres 1, 5