Fig. 16
From: Methods to study organogenesis in decapod crustacean larvae II: analysing cells and tissues
Immunohistochemical localisation of FMRFamide-like neuropeptides in whole mounts of the central nervous system of zoeal stages of Hyas araneus (modified from [138]; see section "Immunohistochemistry" for details). The primary antiserum was visualised with an enzyme-based detection system and the preparations analysed with a bright field microscope (Polyvar, Reichert Jung) and drawn using an attached camera-lucida apparatus to thoroughly analyse the maturation of single neuronal elements AMC: anterior median cell cluster; APN: anterior protocerebral neuropil; CG: commissural ganglion; MD: neuromere of mandible; MX 1, 2: neuromeres of maxilla 1 and 2; T1, 8: thoracic neuromeres 1, 8; P1, P5: pleomeres 1, 5; PPN: posterior protocerebral neuropil; O: oesophageal foramen; OC: oesophageal connective; OL: olfactory lobe; POC: postoesophageal commissure; TCN: tritocerebral neuropil