Fig. 17
From: Methods to study organogenesis in decapod crustacean larvae II: analysing cells and tissues
BrdU labelling of cell nuclei in s-phase in dissected whole mounts of the median brain and ventral nerve cord of C. maenas larvae scanned using a Leica TCS SP5II confocal laser scanning microscope (see section "Using an in vivo marker for cell proliferation" for details). a Median brain, coloured depth coding using the "Z code stack" plugin for ImageJ (R. Meth, unpublished). b Dorsal surface view of a Zoea III median brain (R. Meth, unpublished). b’ Autofluorescence of the right brain hemisphere visualised by UV excitation (R. Meth, unpublished). c Ventral nerve cord of a Megalopa imaged with a Nikon Eclipse 90i upright fluorescence microscope (S. Harzsch, unpublished). d Various containers for immunohistochemistry (image S. Harzsch). d1 Plastic pipettes with drawn-out tips (image S. Harzsch). d2 Petri dish with black Elastosil® coating and micro-forceps. PC: protocerebrum; DC: deutocerebrum; TC: tritocerebrum