Fig. 3
From: Methods to study organogenesis in decapod crustacean larvae II: analysing cells and tissues
Scanning electron microscopy (see section "Scanning electron microscopy (SEM)"). a Accessories for critical point and chemical drying (image R. Melzer). a1 Microporous capsules, self-made specimen holders (Eppendorf tubes with plankton mesh to ensure liquid exchange and prevent loss of larvae), small glass test tubes, and cut-off bases of test tubes to be inserted into microporous capsules. a2 Different types of holders and vials for chemical cleaning and drying. a3 Mounting of specimens: self-adhesive carbon stickers, empty SEM specimen stubs, specimen stub with carbon sticker, specimen stub with “Leit-C-plast”, stub forceps, and two stubs with mounted and gold-sputtered specimens. b Higher magnification of specimen stub with cleaned, dried Zoeae of Porcellana platycheles before sputtering (image R. Meyer, R. Melzer). c “Zoea garden”: Zoea 1 of Panopeus chilensis. d–f SEM pictures of Zoea 1 of Panopeus chilensis, viewed from anterolaterally (d), dorsally (e) and ventrally (f) (R. Meyer, R. Melzer, unpublished)