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Fig. 7 | Helgoland Marine Research

Fig. 7

From: Methods to study organogenesis in decapod crustacean larvae II: analysing cells and tissues

Fig. 7

Frontal semithin sections of a Megalopa of Carcinus maenas (stained according to Holländer and Vaaland 1968, [22, 92]) illustrating the resolution of semi-thin sectioning (F. Spitzner, unpublished; see section "Description of method: plastic embedding, semithin sectioning, and staining with Toluidine Blue and staining according to Holländer and Vaaland" for details). Note that the cuticle (CU) and epidermis are detached (double arrow) due to poor fixation. To avoid such artefacts, adding up to 9% non-electrolytes helps to adjust the osmolarity of the fixative and therefore avoid volume changes of tissue. a Overview section showing the median portion of the pyloric stomach (PS) laterally flanked by the lobes of the digestive gland (DG). b Section through lobes of the digestive gland showing epithelium with the F-, R-, and B-cells surrounding the lumen (LU). c Section showing the median brain with the deutocerebrum (DC) and the dorsally positioned stomatogastric ganglion (STG). The deutocerebrum is characterised by the spherical olfactory lobes (OLs), and the neuropil of which is surrounded by neuronal somata (SO). AA: anterior aorta, B: blister-like cell, F: fibrillary cell, HG: hindgut, MA: mandibular adductor musculature, PS: pyloric stomach, R: resorptive cell, VNC: ventral nerve cord

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