Fig. 8
From: Methods to study organogenesis in decapod crustacean larvae II: analysing cells and tissues
Semithin sectioning using the rotary microtome (images F. Spitzner; see section "Description of method: plastic embedding, semithin sectioning, and staining with Toluidine Blue and staining according to Holländer and Vaaland" for details). a Sample of a Zoea I of Carcinus maenas embedded in Araldite® (black circle). b Clamped embedding block before trimming. c Clamped embedding block after trimming into trapezoid shape to reduce cutting surface and duration of sectioning. d Lifting the section chain out of the water bath. It is useful to reduce the surface tension of the water bath by placing a pipette crosswise in front of the microscope slide when carefully immersing the microscope slide into the water bath (see section "Description of method: plastic embedding, semithin sectioning, and staining with Toluidine Blue and staining according to Holländer and Vaaland" for details)