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Table 1 Summary of speed (from slow* to fast****) and costs (from rather cheap € to rather expensive €€€€) of the methods presented in this paper

From: Methods to study organogenesis in decapod crustacean larvae II: analysing cells and tissues

  Speed Costs of reagents Costs of lab instruments Costs of imaging equipment
Macrophotography ****
Autofluorescence microscopy **** €€/€€€
SEM ** €€ €€ €€€€
Micro CT *** €€ €€€€
Paraplast histology ** €€ €€€ €/€€€
Semithin histology ** €€€ €€€ €/€€€
TEM * €€€ €€€€ €€€€
Immunohistochemistry ** €€€€ €€/€€€€
Proliferation markers ** €€€€ €€/€€€€
Nuclear dyes **** €€ €€/€€€€
  1. Some methods are much more time consuming than others: **** hours, *** one day, ** several days, * several weeks. The costs of reagents summarises necessary chemicals: € < 10, €€ < 100, €€€ < 500, €€€€ < 1,000. The costs of lab instrumentation summarises standard equipment such as orbital shakers, ovens and microtomes: € < 500, €€ < 5,000, €€€ < 10,000, €€€€ < 20,000. For the imaging equipment, some analysis is possible with rather simple microscopes, but more sophisticated instruments (such as a confocal laser scanning microscope) can be rather expensive: € < 2,000, €€ < 20,000, €€€ < 100,000, €€€€ over 250,000.