Methods to study organogenesis in decapod crustacean larvae. I. larval rearing, preparation, and fixation

Torres, G.; Melzer, R. R.; Spitzner, F.; Šargač, Z.; Harzsch, S.; Gimenez, L.

doi:10.1186/s10152-021-00548-x

Helgoland Marine Research

Table 1 Examples for studies on late embryonic and larval organogenesis in representatives of Decapoda and other selected Malacostraca including methodological approaches

From: Methods to study organogenesis in decapod crustacean larvae. I. larval rearing, preparation, and fixation

General external morphology
Compendia with line drawings based on LiMi	[12, 60,61,62]
Hippolyte inermis	clsm, SEM [108]
Carcinus maenas	µCT, 3D [35]
Portunus acuminatus	SEM [32]
Xantho poressa, Xantho pilipes	SEM [109]
Gnathophyllum elegans	SEM [110]
Periclimenes amethysteus	SEM [111]
Several Anomura	EF, LiMi [112]
Several Stomatopoda	EF [113]
Several Polychelida	EF [114]
Hippidae	EF [115]
General anatomy
Hippolyte inermis	clsm, SEM [108]
Cancer anthonyi	PH [116]
Portunus trituberculatus	PH [117]
Carcinus maenas	µCT, 3D [35]
Maja brachydactyla	µCT, 3D [92]
Mouthparts and digestive tract
Several Anomura	EF, LiMi [112]
Maja brachydactyla	µCT, PH, SEM, TEM [118, 119]
Hyas araneus	STH, TEM [84, 120]
Scylla olivacea	PH [121]
Scylla serrata	PH [122,123,124]
Ucides cordatus	PH [125]
Dyspanopeus sayi	SEM, LiMi [126]
Sesarma curacaoense	PH [127]
Several Brachyura	SEM [128]
Ranina ranina	PH [129]
Menippe mercenaria	SEM [130]
Paralithodes camtschaticus	PH [131]
Homarus americanus	TEM, SEM, STH [97, 130, 132]
Astacus astacus, Procambarus fallax f. virginalis	SEM, TEM, PH [133, 134]
Jasus edwardsii	SEM [135]
Macrobrachium amazonicum	SEM [136]
Palaemon elegans	SEM, TEM [34, 136]
Lysmata amboinensis	PH, SEM [137]
Porcellio scaber	STH, SEM, TEM [138]
Osmoregulatory epithelia
Carcinus maenas	IHC, SEM [139], LiMi [140]
Eriocheir sinensis	IHC [141]
Callianassa jamaicense	TEM [142]
Astacus leptodactylus	IHC, PP, SEM [143]
Several Brachyura and Anomala	LiMi [140]
Homarus americanus	IHC, TEM [144]
Palaemontes argentinus	IHC, PH [145, 146]
Litopenaeus stylirostris	IHC, STH [147]
Crangon crangon	IHC, SEM, STH [148]
Antennal glands
Astacus leptodactylus	SEM, TEM, IHC [149, 150]
Homarus gammarus	IHC [151]
Macrobrachium amazonicum	IHC, PP [152, 153]
Palaemontes argentinus	IHC, PP [146]
Integument and tegumental glands
Hyas araneus	STH [120]
Sesarma haematocheir	TEM [154]
Multiple species	STH, TEM [155]
Y organ
Cancer anthonyi	STH [156]
Hyas araneus	STH [120]
Muscle
Several decapod species	IHC [157]
Homarus americanus	IHC [158]
Idothea báltica	IHC [159]
Sensory dorsal organ
Several decapod species	SEM [160]
Eyestalk neuroendocrine centres
Cancer anthonyi	STH [156]
Homarus gammarus	STH, IHC [161, 162]
Compound eyes
Several species	STH, PM, TEM [163, 164]
Carcinus maenas	STH, PM [30]
Hemigrapsus sanguineus	STH [165]
Rhithropanopeus harrisii	STH [165]
Various Anomala	STH [166]
Panulirus longipes	TEM [167]
Procambarus clarkii	STH, TEM [168]
Alima pacifica	3D, osmium-ethyl gallate staining [169]
Aesthetascs
Carcinus maenas	SEM, TEM [170]
Cherax destructor	SEM, LiMi [171]
Structure of the larval CNS and neurogenesis	Embryonic neurogenesis was recently reviewed [172,173,174] and will not be considered here
Carcinus maenas	STH, µCT, 3D, IHC [175,176,177,178]
Hyas araneus	IHC, PM, STH [179,180,181,182,183]
Pachygrapsus marmoratus	STH, 3D [184]
Porcellana platycheles	STH, 3D [184]
Cherax destructor	PM [171, 185, 186]
Homarus americanus	STH, PM, IHC [183, 186,187,188,189,190,191,192]
Homarus gammarus	STH, TEM, IHC [161, 162]
Hippolyte inermis	STH, 3D [184]

clsm: confocal laser-scan microscopy; EF: epifluorescence, IHC: immunohistochemistry; LiMi: light microscopy of external morphology; µCT: X-ray microscopy; PH: paraffin histology; PM: proliferation markers; SEM: scanning electron microscopy; STH: semithin histology; TEM: transmission electron microscopy; 3D: 3D reconstruction

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ISSN: 1438-3888

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