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Absorption of neutral and basic amino acids across the body surface of two annelid species
Absorption von neutralen und basischen Aminosäuren durch die Körperoberfläche zweier Annelidenarten
Helgoländer wissenschaftliche Meeresuntersuchungen volume 28, pages 456–466 (1976)
Kurzfassung
In Versuchen zur Aufnahme verschiedener Aminosäuren durch die Körperoberfläche bei dem OligochaetenEnchytraeus albidus und dem PolychaetenNereis diversicolor betrug die Absorptionsrate von14C-L-Arginin aus einer Umgebungskonzentration von 10 µM nur wenige Prozent der für Glycin und L-Valin bestimmten Werte. Nach einer halbstündigen Versuchsdauer waren neutrale14C-Aminosäuren im Organismus 9- bis 15fach gegenüber dem Medium angereichert, die L-Argininverteilung blieb dagegen unterhalb des Äquilibriums. Die konzentrationsabhängige Aufnahmekurve für L-Arginin bestand beiE. albidus aus einer Diffusions- und einer Sättigungskomponente, die durch andere Aminosäuren hemmbar war. Die stärkste Inhibition erfolgte durch basische Aminosäuren. Eine erhelbiche Hemmung wurde auch durch neutrale Aminosäuren bewirkt. L-Glutamin und L-Asparagin verminderten die L-Argininaufnahme nur geringfügig. Die Gegenwart von sauren,β- undγ-Aminosäuren sowie organischen Säuren und Kohlenhydraten hatte nur einen unbedeutenden oder gar keinen Einfluß zur Folge. Da die L-Argininaufnahme nicht dem Einfluß von Stoffwechselinhibitoren (NaCN und Ouabain) unterlag und Na+-unabhängig war, erfüllt die Sättigungskomponente des L-Arginintransports die Merkmale der “erleichterten Diffusion“. Der Unterschied zu neutralen Aminosäuren, welche aktiv transportiert werden, wird diskutiert.
Summary
1. Absorption of L-arginine across the body surface of the oligachaeteEnchytraeus albidus and the polychaeteNereis diversicolor from 10 µM ambient concentrations proceeds at only few percentages of glycine and L-valine uptake rates. Distribution ratios do not exceed equilibrium. InE. albidus L-lysine is transported at the same low rates as observed for L-arginine.
2. InE. albidus the concentration dependent uptake of L-arginine consits of a saturable component and a diffusion component. The saturable component is susceptible to inhibition by structurally analogous substances. This inhibition is strongest in the presence of other basic amino acids and is still significant in the presence of neutral amino acids. Slight inhibition of L-arginine absorption is caused by L-glutamine and L-asparagine, while inhibition of arginine is only inconsiderable in the presence of acidic andβ- andγ-amino acids. Arginine uptake rates are unchanged by organic acids and carbohydrates.
3. Because of nonsusceptibility of arginine transport to metabolic inhibitors (NaCN and ouabain) and Na+-depletion inE. albidus, the saturable component of arginine uptake is regarded to fulfill the requirements for applying the term “facilitated diffusion”, which is discussed in relation to the active transport of neutral amino acids.
4. The mode of basic amino acid absorption is discussed in comparison to that in other animal species and absorbing tissues.
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Siebers, D. Absorption of neutral and basic amino acids across the body surface of two annelid species. Helgolander Wiss. Meeresunters 28, 456–466 (1976). https://doi.org/10.1007/BF01610593
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DOI: https://doi.org/10.1007/BF01610593